Inhibition of Amyloid beta Protein Fibrillation by Polymeric Nanoparticles
(2008) In Journal of the American Chemical Society 130(46). p.15437-15443- Abstract
- Copolymeric NiPAM:BAM nanoparticles of varying hydrophobicity were found to retard fibrillation of the Alzheimer's disease-associated amyloid beta protein (A beta). We found that these nanoparticles affect mainly the nucleation step of A beta fibrillation. The elongation step is largely unaffected by the particles, and once the M is nucleated, the fibrillation process occurs with the same rate as in the absence of nanoparticles. The extension of the lag phase for fibrillation of A beta is strongly dependent on both the amount and surface character of the nanoparticles. Surface plasmon resonance studies show that A beta binds to the nanoparticles and provide rate and equilibrium constants for the interaction. Numerical analysis of the... (More)
- Copolymeric NiPAM:BAM nanoparticles of varying hydrophobicity were found to retard fibrillation of the Alzheimer's disease-associated amyloid beta protein (A beta). We found that these nanoparticles affect mainly the nucleation step of A beta fibrillation. The elongation step is largely unaffected by the particles, and once the M is nucleated, the fibrillation process occurs with the same rate as in the absence of nanoparticles. The extension of the lag phase for fibrillation of A beta is strongly dependent on both the amount and surface character of the nanoparticles. Surface plasmon resonance studies show that A beta binds to the nanoparticles and provide rate and equilibrium constants for the interaction. Numerical analysis of the kinetic data for fibrillation suggests that binding of monomeric A beta and prefibrillar oligomers to the nanoparticles prevents fibrillation. Moreover, we find that fibrillation of A beta initiated in the absence of nanoparticles can be reversed by addition of nanoparticles up to a particular time point before mature fibrils appear. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1375571
- author
- Cabaleiro-Lago, Celia ; Quinlan-Pluck, Fiona ; Lynch, Iseult ; Lindman, Stina LU ; Minogue, Aedin M. ; Thulin, Eva LU ; Walsh, Dominic M. ; Dawson, Kenneth A. and Linse, Sara LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the American Chemical Society
- volume
- 130
- issue
- 46
- pages
- 15437 - 15443
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000263311300047
- scopus:56449124781
- pmid:18954050
- ISSN
- 1520-5126
- DOI
- 10.1021/ja8041806
- language
- English
- LU publication?
- yes
- id
- 3af8bb12-7a69-4de3-b691-feeba29f1016 (old id 1375571)
- date added to LUP
- 2016-04-01 14:33:54
- date last changed
- 2022-04-22 03:57:24
@article{3af8bb12-7a69-4de3-b691-feeba29f1016, abstract = {{Copolymeric NiPAM:BAM nanoparticles of varying hydrophobicity were found to retard fibrillation of the Alzheimer's disease-associated amyloid beta protein (A beta). We found that these nanoparticles affect mainly the nucleation step of A beta fibrillation. The elongation step is largely unaffected by the particles, and once the M is nucleated, the fibrillation process occurs with the same rate as in the absence of nanoparticles. The extension of the lag phase for fibrillation of A beta is strongly dependent on both the amount and surface character of the nanoparticles. Surface plasmon resonance studies show that A beta binds to the nanoparticles and provide rate and equilibrium constants for the interaction. Numerical analysis of the kinetic data for fibrillation suggests that binding of monomeric A beta and prefibrillar oligomers to the nanoparticles prevents fibrillation. Moreover, we find that fibrillation of A beta initiated in the absence of nanoparticles can be reversed by addition of nanoparticles up to a particular time point before mature fibrils appear.}}, author = {{Cabaleiro-Lago, Celia and Quinlan-Pluck, Fiona and Lynch, Iseult and Lindman, Stina and Minogue, Aedin M. and Thulin, Eva and Walsh, Dominic M. and Dawson, Kenneth A. and Linse, Sara}}, issn = {{1520-5126}}, language = {{eng}}, number = {{46}}, pages = {{15437--15443}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of the American Chemical Society}}, title = {{Inhibition of Amyloid beta Protein Fibrillation by Polymeric Nanoparticles}}, url = {{http://dx.doi.org/10.1021/ja8041806}}, doi = {{10.1021/ja8041806}}, volume = {{130}}, year = {{2008}}, }