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Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation

Paquin, Raphael ; Ferrage, Fabien ; Mulder, Frans LU ; Akke, Mikael LU orcid and Bodenhausen, Geoffrey (2008) In Journal of the American Chemical Society 130(47). p.15805-15805
Abstract
Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
130
issue
47
pages
15805 - 15805
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000263319600031
  • scopus:56749180963
  • pmid:18975903
ISSN
1520-5126
DOI
10.1021/ja803794g
language
English
LU publication?
yes
id
e015a054-3489-4343-8ea9-5aee733962f5 (old id 1375592)
date added to LUP
2016-04-01 14:25:12
date last changed
2025-10-14 10:15:57
@article{e015a054-3489-4343-8ea9-5aee733962f5,
  abstract     = {{Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.}},
  author       = {{Paquin, Raphael and Ferrage, Fabien and Mulder, Frans and Akke, Mikael and Bodenhausen, Geoffrey}},
  issn         = {{1520-5126}},
  language     = {{eng}},
  number       = {{47}},
  pages        = {{15805--15805}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of the American Chemical Society}},
  title        = {{Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation}},
  url          = {{http://dx.doi.org/10.1021/ja803794g}},
  doi          = {{10.1021/ja803794g}},
  volume       = {{130}},
  year         = {{2008}},
}