Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals
(2004) In Bioelectrochemistry 63(1-2). p.277-280- Abstract
- The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the... (More)
- The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138522
- author
- Shipovskov, Stepan LU ; Ferapontova, Elena LU ; Gazaryan, I and Ruzgas, Tautgirdas LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bi-electrode system, Recombinant horseradish peroxidase, Cytochrome c, Hydrogen peroxide, Superoxide radical
- in
- Bioelectrochemistry
- volume
- 63
- issue
- 1-2
- pages
- 277 - 280
- publisher
- Elsevier
- external identifiers
-
- wos:000221786900050
- pmid:15110287
- scopus:2042456719
- pmid:15110287
- ISSN
- 1878-562X
- DOI
- 10.1016/j.bioelechem.2003.09.026
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
- id
- a874e76e-3f7d-4ee2-8739-5cbdfe327fd9 (old id 138522)
- date added to LUP
- 2016-04-01 11:46:16
- date last changed
- 2022-04-05 04:50:07
@article{a874e76e-3f7d-4ee2-8739-5cbdfe327fd9, abstract = {{The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved.}}, author = {{Shipovskov, Stepan and Ferapontova, Elena and Gazaryan, I and Ruzgas, Tautgirdas}}, issn = {{1878-562X}}, keywords = {{Bi-electrode system; Recombinant horseradish peroxidase; Cytochrome c; Hydrogen peroxide; Superoxide radical}}, language = {{eng}}, number = {{1-2}}, pages = {{277--280}}, publisher = {{Elsevier}}, series = {{Bioelectrochemistry}}, title = {{Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals}}, url = {{http://dx.doi.org/10.1016/j.bioelechem.2003.09.026}}, doi = {{10.1016/j.bioelechem.2003.09.026}}, volume = {{63}}, year = {{2004}}, }