Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale
(2004) In Applied Biochemistry and Biotechnology 114(1-3). p.601-617- Abstract
- Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the... (More)
- Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138861
- author
- Rudolf, Andreas LU ; Galbe, Mats LU and Lidén, Gunnar LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Biochemistry and Biotechnology
- volume
- 114
- issue
- 1-3
- pages
- 601 - 617
- publisher
- Humana Press
- external identifiers
-
- wos:000221186200050
- scopus:11244315135
- ISSN
- 1559-0291
- DOI
- 10.1385/ABAB:114:1-3:601
- language
- English
- LU publication?
- yes
- id
- 51d38725-f2f9-4c60-ae40-ed953f6d56dd (old id 138861)
- date added to LUP
- 2016-04-01 11:33:30
- date last changed
- 2023-11-10 16:43:46
@article{51d38725-f2f9-4c60-ae40-ed953f6d56dd, abstract = {{Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.}}, author = {{Rudolf, Andreas and Galbe, Mats and Lidén, Gunnar}}, issn = {{1559-0291}}, language = {{eng}}, number = {{1-3}}, pages = {{601--617}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology}}, title = {{Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale}}, url = {{http://dx.doi.org/10.1385/ABAB:114:1-3:601}}, doi = {{10.1385/ABAB:114:1-3:601}}, volume = {{114}}, year = {{2004}}, }