Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood
(2009) 7th International Conference on Scientific and Clinical Applications of Magnetic Carriers 321(10). p.1632-1634- Abstract
- We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed... (More)
- We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay. (C) 2009 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1400211
- author
- Ibraimi, Filiz LU ; Kriz, Kirstin LU ; Merin, Henrik and Kriz, Dario
- organization
- publishing date
- 2009
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Veterinary clinics, Diagnostic test, Two-site immunoassay, nanoparticles, Superparamagnetic, Canine C-reactive protein, Magnetic permeability
- host publication
- Journal Of Magnetism And Magnetic Materials
- volume
- 321
- issue
- 10
- pages
- 1632 - 1634
- publisher
- Elsevier
- conference name
- 7th International Conference on Scientific and Clinical Applications of Magnetic Carriers
- conference dates
- 2008-05-20 - 2008-05-24
- external identifiers
-
- wos:000265278000068
- scopus:64249131409
- ISSN
- 0304-8853
- DOI
- 10.1016/j.jmmm.2009.02.102
- language
- English
- LU publication?
- yes
- id
- 3b825e38-895c-4e04-9c37-02614972db6b (old id 1400211)
- date added to LUP
- 2016-04-01 14:31:06
- date last changed
- 2022-01-28 01:02:54
@inproceedings{3b825e38-895c-4e04-9c37-02614972db6b, abstract = {{We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay. (C) 2009 Elsevier B.V. All rights reserved.}}, author = {{Ibraimi, Filiz and Kriz, Kirstin and Merin, Henrik and Kriz, Dario}}, booktitle = {{Journal Of Magnetism And Magnetic Materials}}, issn = {{0304-8853}}, keywords = {{Veterinary clinics; Diagnostic test; Two-site immunoassay; nanoparticles; Superparamagnetic; Canine C-reactive protein; Magnetic permeability}}, language = {{eng}}, number = {{10}}, pages = {{1632--1634}}, publisher = {{Elsevier}}, title = {{Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood}}, url = {{http://dx.doi.org/10.1016/j.jmmm.2009.02.102}}, doi = {{10.1016/j.jmmm.2009.02.102}}, volume = {{321}}, year = {{2009}}, }