Agarose-coated anion exchanger prevents cell-adsorbent interactions
(2004) In Journal of Chromatography A 1043(2). p.195-200- Abstract
- A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The... (More)
- A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140627
- author
- Viloria-Cols, Maria LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fluidized bed adsorption, Fouling, Agarose coating, Amberlite IRA-400, Cells, Adsorbents, Lactic acid
- in
- Journal of Chromatography A
- volume
- 1043
- issue
- 2
- pages
- 195 - 200
- publisher
- Elsevier
- external identifiers
-
- wos:000222807500008
- pmid:15330092
- scopus:3042839749
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2004.05.086
- language
- English
- LU publication?
- yes
- id
- 05fd99aa-69a1-466a-90f8-7fa1129671ce (old id 140627)
- date added to LUP
- 2016-04-01 15:46:59
- date last changed
- 2022-02-12 17:33:17
@article{05fd99aa-69a1-466a-90f8-7fa1129671ce,
abstract = {{A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent. (C) 2004 Elsevier B.V. All rights reserved.}},
author = {{Viloria-Cols, Maria and Hatti-Kaul, Rajni and Mattiasson, Bo}},
issn = {{0021-9673}},
keywords = {{Fluidized bed adsorption; Fouling; Agarose coating; Amberlite IRA-400; Cells; Adsorbents; Lactic acid}},
language = {{eng}},
number = {{2}},
pages = {{195--200}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Agarose-coated anion exchanger prevents cell-adsorbent interactions}},
url = {{http://dx.doi.org/10.1016/j.chroma.2004.05.086}},
doi = {{10.1016/j.chroma.2004.05.086}},
volume = {{1043}},
year = {{2004}},
}