Investigation of electron transfer between cellobiose dehydrogenase from Myriococcum Thermophilum and gold electrodes
(2007) In Chemical Analysis 52(6). p.945-960- Abstract
- Cellobiose dehydrogenase (CDH) is a monomeric protein consisting of two subdomains: a larger flavin-associated domain (DHcdh) and a smaller heme-binding domain (CYTcdh), connected via a protease cleavable linker region. In this study, the inter-domain electron transfer, using the CDH from the ascomycete fungus Myriococcum thermophilum and thiol (SAM) modified gold electrodes, was investigated with cyclic voltammetry and UV-VIS spectroelectrochemistry. The effect of the SAM and pH on the formal potential of the heme domain of CDH and on the current generated by the electrocatalytic oxidation of cellobiose and lactose was evaluated with voltammetric techniques. The oxidation-reduction midpoint potentials of the DHcdh, CYTcdh, and whole CDH... (More)
- Cellobiose dehydrogenase (CDH) is a monomeric protein consisting of two subdomains: a larger flavin-associated domain (DHcdh) and a smaller heme-binding domain (CYTcdh), connected via a protease cleavable linker region. In this study, the inter-domain electron transfer, using the CDH from the ascomycete fungus Myriococcum thermophilum and thiol (SAM) modified gold electrodes, was investigated with cyclic voltammetry and UV-VIS spectroelectrochemistry. The effect of the SAM and pH on the formal potential of the heme domain of CDH and on the current generated by the electrocatalytic oxidation of cellobiose and lactose was evaluated with voltammetric techniques. The oxidation-reduction midpoint potentials of the DHcdh, CYTcdh, and whole CDH unit were estimated at different pH values using a long-optical-pathway thin capillary-type spectroelectrochemical cell. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1407572
- author
- Coman, Vasile LU ; Harreither, Wolfgang ; Ludwig, Roland ; Haltrich, Dietmar and Gorton, Lo
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- electrode, thiol modified gold, cellobiose dehydrogenase, Myriococcum thermophilum, direct electron transfer, bioelectrocatalysis
- in
- Chemical Analysis
- volume
- 52
- issue
- 6
- pages
- 945 - 960
- publisher
- Polskie Towarzystwo Chemiczne - Polish Chemical Society
- external identifiers
-
- wos:000252951200007
- scopus:39649094088
- ISSN
- 0009-2223
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- c1b7558a-f7e0-4fb9-9d6c-f73fd9a6fa4c (old id 1407572)
- date added to LUP
- 2016-04-01 17:06:29
- date last changed
- 2022-01-29 00:23:03
@article{c1b7558a-f7e0-4fb9-9d6c-f73fd9a6fa4c,
abstract = {{Cellobiose dehydrogenase (CDH) is a monomeric protein consisting of two subdomains: a larger flavin-associated domain (DHcdh) and a smaller heme-binding domain (CYTcdh), connected via a protease cleavable linker region. In this study, the inter-domain electron transfer, using the CDH from the ascomycete fungus Myriococcum thermophilum and thiol (SAM) modified gold electrodes, was investigated with cyclic voltammetry and UV-VIS spectroelectrochemistry. The effect of the SAM and pH on the formal potential of the heme domain of CDH and on the current generated by the electrocatalytic oxidation of cellobiose and lactose was evaluated with voltammetric techniques. The oxidation-reduction midpoint potentials of the DHcdh, CYTcdh, and whole CDH unit were estimated at different pH values using a long-optical-pathway thin capillary-type spectroelectrochemical cell.}},
author = {{Coman, Vasile and Harreither, Wolfgang and Ludwig, Roland and Haltrich, Dietmar and Gorton, Lo}},
issn = {{0009-2223}},
keywords = {{electrode; thiol modified gold; cellobiose dehydrogenase; Myriococcum thermophilum; direct electron transfer; bioelectrocatalysis}},
language = {{eng}},
number = {{6}},
pages = {{945--960}},
publisher = {{Polskie Towarzystwo Chemiczne - Polish Chemical Society}},
series = {{Chemical Analysis}},
title = {{Investigation of electron transfer between cellobiose dehydrogenase from Myriococcum Thermophilum and gold electrodes}},
volume = {{52}},
year = {{2007}},
}