Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes
(2018) In Histochemistry and Cell Biology 149(5). p.503-516- Abstract
Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration... (More)
Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm2 fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.
(Less)
- author
- organization
- publishing date
- 2018-02-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cancer microenvironment, Cancer-associated fibroblasts, Chemokine, Cytokine, Keratinocytes, Melanoma
- in
- Histochemistry and Cell Biology
- volume
- 149
- issue
- 5
- pages
- 503 - 516
- publisher
- Springer
- external identifiers
-
- pmid:29435761
- scopus:85041895906
- ISSN
- 0948-6143
- DOI
- 10.1007/s00418-018-1650-4
- language
- English
- LU publication?
- yes
- id
- 140d43d9-027f-4380-abec-c47b999ae257
- date added to LUP
- 2018-03-01 10:30:24
- date last changed
- 2024-08-05 13:38:46
@article{140d43d9-027f-4380-abec-c47b999ae257, abstract = {{<p>Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm<sup>2</sup> fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.</p>}}, author = {{Jobe, Njainday Pulo and Živicová, Veronika and Mifková, Alžběta and Rösel, Daniel and Dvořánková, Barbora and Kodet, Ondřej and Strnad, Hynek and Kolář, Michal and Šedo, Aleksi and Smetana, Karel and Strnadová, Karolina and Brábek, Jan and Lacina, Lukáš}}, issn = {{0948-6143}}, keywords = {{Cancer microenvironment; Cancer-associated fibroblasts; Chemokine; Cytokine; Keratinocytes; Melanoma}}, language = {{eng}}, month = {{02}}, number = {{5}}, pages = {{503--516}}, publisher = {{Springer}}, series = {{Histochemistry and Cell Biology}}, title = {{Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes}}, url = {{http://dx.doi.org/10.1007/s00418-018-1650-4}}, doi = {{10.1007/s00418-018-1650-4}}, volume = {{149}}, year = {{2018}}, }