Ultrastructural cartilage abnormalities in MIA/CD-RAP-deficient mice
(2002) In Molecular and Cellular Biology 22(5). p.1438-1445- Abstract
- MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were... (More)
- MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were viable and developed normally, and histological examination of the organs by means of light microscopy revealed no major abnormalities. In contrast, electron microscopic studies of cartilage composition revealed subtle defects in collagen fiber density, diameter, and arrangement, as well as changes in the number and morphology of chondrocytic microvilli. Taken together, our data indicate that MIA/CD-RAP is essentially required for formation of the highly ordered ultrastructural fiber architecture in cartilage and may have a role in regulating chondrocyte matrix interactions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141957
- author
- Moser, Markus ; Bosserhoff, Anja-Katrin ; Hunziker, Ernst B. ; Sandell, Linda ; Fässler, Reinhard LU and Buettner, Reinhard
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Biology
- volume
- 22
- issue
- 5
- pages
- 1438 - 1445
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:11839810
- wos:000173837000016
- scopus:0036172080
- ISSN
- 0270-7306
- DOI
- 10.1128/MCB.22.5.1438-1445.2002
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- 1eec37b8-4125-49dc-930f-86e86c3dcf08 (old id 141957)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11839810&query_hl=132
- date added to LUP
- 2016-04-01 11:32:50
- date last changed
- 2022-01-26 06:57:58
@article{1eec37b8-4125-49dc-930f-86e86c3dcf08,
abstract = {{MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were viable and developed normally, and histological examination of the organs by means of light microscopy revealed no major abnormalities. In contrast, electron microscopic studies of cartilage composition revealed subtle defects in collagen fiber density, diameter, and arrangement, as well as changes in the number and morphology of chondrocytic microvilli. Taken together, our data indicate that MIA/CD-RAP is essentially required for formation of the highly ordered ultrastructural fiber architecture in cartilage and may have a role in regulating chondrocyte matrix interactions.}},
author = {{Moser, Markus and Bosserhoff, Anja-Katrin and Hunziker, Ernst B. and Sandell, Linda and Fässler, Reinhard and Buettner, Reinhard}},
issn = {{0270-7306}},
language = {{eng}},
number = {{5}},
pages = {{1438--1445}},
publisher = {{American Society for Microbiology}},
series = {{Molecular and Cellular Biology}},
title = {{Ultrastructural cartilage abnormalities in MIA/CD-RAP-deficient mice}},
url = {{https://lup.lub.lu.se/search/files/2538592/624801.pdf}},
doi = {{10.1128/MCB.22.5.1438-1445.2002}},
volume = {{22}},
year = {{2002}},
}