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Immuno-arrays for multianalyte analysis of chlorotriazines

Bhand, Sunil LU ; Surugiu, Ioana ; Dzgoev, Anatoly LU ; Ramanathan, Kumaran LU ; Sundaram, P V and Danielsson, Bengt LU (2005) In Talanta 65(2). p.331-336
Abstract
In this paper, a novel strategy for multicomponent analysis of two classes of pesticides such as triazines (atrazine and simazine) and phenoxyalkanoic acids (2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 4-chlorophenoxyacetic acid (CPOAc), phenoxyacetic acid (POAc)) employing immuno-arrays is demonstrated. The approach is based on cross-reactive arrays of specific antibody pairs coupled to chemometric pattern recognition. The monoclonal antibody pairs employed in this work (atrazine–simazine and 2,4-D) are specific towards a set of analytes and preclude a particular set of others present in the sample matrix. Antibody pairs of atrazine, simazine, and 2,4-D are used to discriminate and quantify... (More)
In this paper, a novel strategy for multicomponent analysis of two classes of pesticides such as triazines (atrazine and simazine) and phenoxyalkanoic acids (2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 4-chlorophenoxyacetic acid (CPOAc), phenoxyacetic acid (POAc)) employing immuno-arrays is demonstrated. The approach is based on cross-reactive arrays of specific antibody pairs coupled to chemometric pattern recognition. The monoclonal antibody pairs employed in this work (atrazine–simazine and 2,4-D) are specific towards a set of analytes and preclude a particular set of others present in the sample matrix. Antibody pairs of atrazine, simazine, and 2,4-D are used to discriminate and quantify analyte of interest. Atrazine was quantified in presence of trace concentration of simazine and that of 2,4-D. The combinatorial cross-reactivity of antibody pairs towards simazine, atrazine and 2,4-D is used to distinguish among different classes of analytes and their influence on the signal suppression in immuno-techniques. These sensors exclude recognition by carbamates such as carbaryl and carbofuran. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Talanta
volume
65
issue
2
pages
331 - 336
publisher
Elsevier
external identifiers
  • wos:000225381900006
  • scopus:8444227044
ISSN
1873-3573
DOI
10.1016/j.talanta.2004.07.009
language
English
LU publication?
yes
id
cc6cfeab-5e45-4a0f-b430-51f5e1e3c58d (old id 142509)
date added to LUP
2016-04-01 16:22:16
date last changed
2022-01-28 19:12:21
@article{cc6cfeab-5e45-4a0f-b430-51f5e1e3c58d,
  abstract     = {{In this paper, a novel strategy for multicomponent analysis of two classes of pesticides such as triazines (atrazine and simazine) and phenoxyalkanoic acids (2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 4-chlorophenoxyacetic acid (CPOAc), phenoxyacetic acid (POAc)) employing immuno-arrays is demonstrated. The approach is based on cross-reactive arrays of specific antibody pairs coupled to chemometric pattern recognition. The monoclonal antibody pairs employed in this work (atrazine–simazine and 2,4-D) are specific towards a set of analytes and preclude a particular set of others present in the sample matrix. Antibody pairs of atrazine, simazine, and 2,4-D are used to discriminate and quantify analyte of interest. Atrazine was quantified in presence of trace concentration of simazine and that of 2,4-D. The combinatorial cross-reactivity of antibody pairs towards simazine, atrazine and 2,4-D is used to distinguish among different classes of analytes and their influence on the signal suppression in immuno-techniques. These sensors exclude recognition by carbamates such as carbaryl and carbofuran.}},
  author       = {{Bhand, Sunil and Surugiu, Ioana and Dzgoev, Anatoly and Ramanathan, Kumaran and Sundaram, P V and Danielsson, Bengt}},
  issn         = {{1873-3573}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{331--336}},
  publisher    = {{Elsevier}},
  series       = {{Talanta}},
  title        = {{Immuno-arrays for multianalyte analysis of chlorotriazines}},
  url          = {{http://dx.doi.org/10.1016/j.talanta.2004.07.009}},
  doi          = {{10.1016/j.talanta.2004.07.009}},
  volume       = {{65}},
  year         = {{2005}},
}