Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.
(2005) In Experimental Neurology 196(2). p.373-380- Abstract
- Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell... (More)
- Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell survival and astrocyte proliferation. Our results suggest that PA6 cells do not have a general capacity to promote differentiation into dopaminergic neurons from all types of stem cells, but that they may specifically induce dopaminergic differentiation of highly uncommitted stem cells such as embryonic stem cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/144922
- author
- Roybon, Laurent LU ; Brundin, Patrik LU and Li, Jia-Yi LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- astrocytes, dopaminergic neurons, neurosphere, PA6 stromal cell, neural stem cells
- in
- Experimental Neurology
- volume
- 196
- issue
- 2
- pages
- 373 - 380
- publisher
- Elsevier
- external identifiers
-
- wos:000233547700018
- pmid:16199035
- scopus:27744478490
- ISSN
- 0014-4886
- DOI
- 10.1016/j.expneurol.2005.08.023
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Neurobiology (013212024)
- id
- 782ab644-07a1-48b3-8c0a-087f1d9e6be0 (old id 144922)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16199035&dopt=Abstract
- date added to LUP
- 2016-04-01 12:38:15
- date last changed
- 2022-01-27 07:50:23
@article{782ab644-07a1-48b3-8c0a-087f1d9e6be0,
abstract = {{Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell survival and astrocyte proliferation. Our results suggest that PA6 cells do not have a general capacity to promote differentiation into dopaminergic neurons from all types of stem cells, but that they may specifically induce dopaminergic differentiation of highly uncommitted stem cells such as embryonic stem cells.}},
author = {{Roybon, Laurent and Brundin, Patrik and Li, Jia-Yi}},
issn = {{0014-4886}},
keywords = {{astrocytes; dopaminergic neurons; neurosphere; PA6 stromal cell; neural stem cells}},
language = {{eng}},
number = {{2}},
pages = {{373--380}},
publisher = {{Elsevier}},
series = {{Experimental Neurology}},
title = {{Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.}},
url = {{http://dx.doi.org/10.1016/j.expneurol.2005.08.023}},
doi = {{10.1016/j.expneurol.2005.08.023}},
volume = {{196}},
year = {{2005}},
}