Two novel IgG endopeptidases of Streptococcus equi
(2009) In FEMS Microbiology Letters 298(1). p.44-50- Abstract
- Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep... (More)
- Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1461631
- author
- Hulting, Greta ; Flock, Margareta ; Frykberg, Lars ; Lannergård, Jonas LU ; Flock, Jan-Ingmar and Guss, Bengt
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- vaccine, IgG endopeptidases, IdeS, IdeZ, Streptococcus equi, IdeE
- in
- FEMS Microbiology Letters
- volume
- 298
- issue
- 1
- pages
- 44 - 50
- publisher
- Oxford University Press
- external identifiers
-
- wos:000268170700006
- scopus:68149128414
- pmid:19659725
- ISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.2009.01698.x
- language
- English
- LU publication?
- yes
- id
- e623d9fe-8e34-4354-8435-b0617211e392 (old id 1461631)
- date added to LUP
- 2016-04-01 13:42:04
- date last changed
- 2022-03-14 01:26:08
@article{e623d9fe-8e34-4354-8435-b0617211e392, abstract = {{Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection.}}, author = {{Hulting, Greta and Flock, Margareta and Frykberg, Lars and Lannergård, Jonas and Flock, Jan-Ingmar and Guss, Bengt}}, issn = {{1574-6968}}, keywords = {{vaccine; IgG endopeptidases; IdeS; IdeZ; Streptococcus equi; IdeE}}, language = {{eng}}, number = {{1}}, pages = {{44--50}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Two novel IgG endopeptidases of Streptococcus equi}}, url = {{http://dx.doi.org/10.1111/j.1574-6968.2009.01698.x}}, doi = {{10.1111/j.1574-6968.2009.01698.x}}, volume = {{298}}, year = {{2009}}, }