The cGMP system in normal and degenerating mouse neuroretina : New proteins with cGMP interaction potential identified by a proteomics approach
(2021) In Journal of Neurochemistry 157(6). p.2173-2186- Abstract
The hereditary disease Retinitis pigmentosa results in severe vision loss due to photoreceptor degeneration by unclear mechanisms. In several disease models, the second messenger cGMP accumulates in the degenerating photoreceptors, where it may over-activate specific cGMP-interacting proteins, like cGMP-dependent protein kinase. Moreover, interventions that counteract the activity of these proteins lead to reduced photoreceptor cell death. Yet there is little or no information whether other than such regular cGMP-interactors are present in the retina, which we, therefore, investigated in wild-type and retinal degeneration (rd1, rd10, and rd2) mouse models. An affinity chromatography based proteomics approach that utilized immobilized... (More)
The hereditary disease Retinitis pigmentosa results in severe vision loss due to photoreceptor degeneration by unclear mechanisms. In several disease models, the second messenger cGMP accumulates in the degenerating photoreceptors, where it may over-activate specific cGMP-interacting proteins, like cGMP-dependent protein kinase. Moreover, interventions that counteract the activity of these proteins lead to reduced photoreceptor cell death. Yet there is little or no information whether other than such regular cGMP-interactors are present in the retina, which we, therefore, investigated in wild-type and retinal degeneration (rd1, rd10, and rd2) mouse models. An affinity chromatography based proteomics approach that utilized immobilized cGMP analogs was applied to enrich and select for regular and potentially new cGMP-interacting proteins as identified by mass spectrometry. This approach revealed 12 regular and ten potentially new retinal cGMP-interacting proteins (e.g., EPAC2 and CaMKIIα). Several of the latter were found to be expressed in the photoreceptors and to have proximity to cGMP and may thus be of interest when defining prospective therapeutic targets or biomarkers for retinal degeneration.
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- author
- Rasmussen, Michel LU ; Welinder, Charlotte LU ; Schwede, Frank and Ekström, Per LU
- organization
- publishing date
- 2021-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chemical proteomics, Photoreceptors, Retinal degeneration, cGMP, cGMP-interacting proteins
- in
- Journal of Neurochemistry
- volume
- 157
- issue
- 6
- pages
- 14 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:33230839
- scopus:85097070898
- ISSN
- 1471-4159
- DOI
- 10.1111/jnc.15251
- language
- English
- LU publication?
- yes
- additional info
- This article is protected by copyright. All rights reserved.
- id
- 14ac344a-6ea2-4983-997c-30ac821f6747
- date added to LUP
- 2020-11-26 14:01:28
- date last changed
- 2024-06-27 02:19:09
@article{14ac344a-6ea2-4983-997c-30ac821f6747, abstract = {{<p>The hereditary disease Retinitis pigmentosa results in severe vision loss due to photoreceptor degeneration by unclear mechanisms. In several disease models, the second messenger cGMP accumulates in the degenerating photoreceptors, where it may over-activate specific cGMP-interacting proteins, like cGMP-dependent protein kinase. Moreover, interventions that counteract the activity of these proteins lead to reduced photoreceptor cell death. Yet there is little or no information whether other than such regular cGMP-interactors are present in the retina, which we, therefore, investigated in wild-type and retinal degeneration (rd1, rd10, and rd2) mouse models. An affinity chromatography based proteomics approach that utilized immobilized cGMP analogs was applied to enrich and select for regular and potentially new cGMP-interacting proteins as identified by mass spectrometry. This approach revealed 12 regular and ten potentially new retinal cGMP-interacting proteins (e.g., EPAC2 and CaMKIIα). Several of the latter were found to be expressed in the photoreceptors and to have proximity to cGMP and may thus be of interest when defining prospective therapeutic targets or biomarkers for retinal degeneration.</p>}}, author = {{Rasmussen, Michel and Welinder, Charlotte and Schwede, Frank and Ekström, Per}}, issn = {{1471-4159}}, keywords = {{Chemical proteomics; Photoreceptors; Retinal degeneration; cGMP; cGMP-interacting proteins}}, language = {{eng}}, month = {{06}}, number = {{6}}, pages = {{2173--2186}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of Neurochemistry}}, title = {{The cGMP system in normal and degenerating mouse neuroretina : New proteins with cGMP interaction potential identified by a proteomics approach}}, url = {{https://lup.lub.lu.se/search/files/90756456/The_cGMP_system_in_normal_and_degenerating_mouse_neuroretina_New_proteins_with_cGMP_interaction_potential_identified_by_a_proteomics_approach.pdf}}, doi = {{10.1111/jnc.15251}}, volume = {{157}}, year = {{2021}}, }