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Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration

Masoumi, Katarzyna Chmielarska LU ; Huang, Xiaoli LU ; Sime, Wondossen LU ; Mirkov, Anna ; Munksgaard Thorén, Matilda ; Massoumi, Ramin LU and Lundgren-Åkerlund, Evy LU (2021) In Cancers 13(5).
Abstract

Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies... (More)

Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies significantly suppressed GB tumor growth compared to control antibodies. Immunohistochemical analysis of the GB tumors showed lower expression of the proliferation marker Ki67 and an increased expression of cleaved caspase-3 after treatment with integrin α10 antibodies, further supporting a therapeutic effect. Our results suggest that function-blocking antibody targeting integrin α10β1 is a promising therapeutic strategy for the treatment of glioblastoma.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Cancers
volume
13
issue
5
article number
1184
publisher
MDPI AG
external identifiers
  • pmid:33803359
  • scopus:85102109465
ISSN
2072-6694
DOI
10.3390/cancers13051184
language
English
LU publication?
no
id
14dc8382-f64b-42d8-ad39-61cad8ab8319
date added to LUP
2024-09-30 20:16:48
date last changed
2025-04-15 23:25:37
@article{14dc8382-f64b-42d8-ad39-61cad8ab8319,
  abstract     = {{<p>Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies significantly suppressed GB tumor growth compared to control antibodies. Immunohistochemical analysis of the GB tumors showed lower expression of the proliferation marker Ki67 and an increased expression of cleaved caspase-3 after treatment with integrin α10 antibodies, further supporting a therapeutic effect. Our results suggest that function-blocking antibody targeting integrin α10β1 is a promising therapeutic strategy for the treatment of glioblastoma.</p>}},
  author       = {{Masoumi, Katarzyna Chmielarska and Huang, Xiaoli and Sime, Wondossen and Mirkov, Anna and Munksgaard Thorén, Matilda and Massoumi, Ramin and Lundgren-Åkerlund, Evy}},
  issn         = {{2072-6694}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{5}},
  publisher    = {{MDPI AG}},
  series       = {{Cancers}},
  title        = {{Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration}},
  url          = {{http://dx.doi.org/10.3390/cancers13051184}},
  doi          = {{10.3390/cancers13051184}},
  volume       = {{13}},
  year         = {{2021}},
}