Marked upregulation of cholesterol 25-hydroxylase expression by lipopolysaccharide
Diczfalusy, Ulf ; Berg, Katarina LU ; Carlsson, Ann-Margreth LU ; Gong, Mei ; Golenbock, Douglas T. ; Rooyackers, Olav ; Flaring, Urban and Björkbacka, Harry LU
(2009)
In Journal of Lipid Research
50(11).
p.2258-2264
- Abstract
- During screening of genes upregulated by lipopolysaccharide (LPS; endotoxin) treatment of bone marrow-derived mouse macrophages, it was unexpectedly found that cholesterol 25-hydroxylase (Ch25h) was strongly upregulated. Treatment of macrophages with 10 ng/ml of LPS for 2 h resulted in a 35-fold increase in the expression of Ch25h. In contrast, LPS treatment did not increase the expression of Cyp27a1 or Cyp7b1. The increased Ch25h expression was found to be independent of Myeloid differentiation protein 88 signaling but dependent on Toll-like receptor 4 signaling. LPS treatment of macrophages caused a 6- to 7-fold increase in cellular 25-hydroxycholesterol concentration. When macrophages were treated with increasing concentrations of... (More)
- During screening of genes upregulated by lipopolysaccharide (LPS; endotoxin) treatment of bone marrow-derived mouse macrophages, it was unexpectedly found that cholesterol 25-hydroxylase (Ch25h) was strongly upregulated. Treatment of macrophages with 10 ng/ml of LPS for 2 h resulted in a 35-fold increase in the expression of Ch25h. In contrast, LPS treatment did not increase the expression of Cyp27a1 or Cyp7b1. The increased Ch25h expression was found to be independent of Myeloid differentiation protein 88 signaling but dependent on Toll-like receptor 4 signaling. LPS treatment of macrophages caused a 6- to 7-fold increase in cellular 25-hydroxycholesterol concentration. When macrophages were treated with increasing concentrations of 25-hydroxycholesterol, a dose-dependent release of CCL5 into the culture medium was observed. Intravenous injection of LPS in eight healthy volunteers resulted in an increase in plasma 25-hydroxycholesterol concentration. The possibility is discussed that 25-hydroxycholesterol may have a role in the inflammatory response, in addition to its more established role in the regulation of cholesterol homeostasis.-Diczfalusy, U., K. E. Olofsson, A- M. Carlsson, M. Gong, D. T. Golenbock, O. Rooyackers, U. Flaring, and H. Bjorkbacka. Marked upregulation of cholesterol 25-hydroxylase expression by lipopolysaccharide. J. Lipids Res. 2009. 50: 2258-2264. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1506271
- author
- Diczfalusy, Ulf
; Berg, Katarina
LU
; Carlsson, Ann-Margreth
LU
; Gong, Mei
; Golenbock, Douglas T.
; Rooyackers, Olav
; Flaring, Urban
and Björkbacka, Harry
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- sepsis, inflammation, endotoxin, 25-hydroxycholesterol, oxysterols, macrophages
- in
- Journal of Lipid Research
- volume
- 50
- issue
- 11
- pages
- 2258 - 2264
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000270860900014
- scopus:70349757041
- pmid:19502589
- ISSN
- 1539-7262
- DOI
- 10.1194/jlr.M900107-JLR200
- language
- English
- LU publication?
- yes
- id
- ce8ed87d-6cc5-4d3f-b4ef-d94a235c5b96 (old id 1506271)
- date added to LUP
- 2016-04-01 12:05:20
- date last changed
- 2025-04-04 13:57:07
@article{ce8ed87d-6cc5-4d3f-b4ef-d94a235c5b96,
abstract = {{During screening of genes upregulated by lipopolysaccharide (LPS; endotoxin) treatment of bone marrow-derived mouse macrophages, it was unexpectedly found that cholesterol 25-hydroxylase (Ch25h) was strongly upregulated. Treatment of macrophages with 10 ng/ml of LPS for 2 h resulted in a 35-fold increase in the expression of Ch25h. In contrast, LPS treatment did not increase the expression of Cyp27a1 or Cyp7b1. The increased Ch25h expression was found to be independent of Myeloid differentiation protein 88 signaling but dependent on Toll-like receptor 4 signaling. LPS treatment of macrophages caused a 6- to 7-fold increase in cellular 25-hydroxycholesterol concentration. When macrophages were treated with increasing concentrations of 25-hydroxycholesterol, a dose-dependent release of CCL5 into the culture medium was observed. Intravenous injection of LPS in eight healthy volunteers resulted in an increase in plasma 25-hydroxycholesterol concentration. The possibility is discussed that 25-hydroxycholesterol may have a role in the inflammatory response, in addition to its more established role in the regulation of cholesterol homeostasis.-Diczfalusy, U., K. E. Olofsson, A- M. Carlsson, M. Gong, D. T. Golenbock, O. Rooyackers, U. Flaring, and H. Bjorkbacka. Marked upregulation of cholesterol 25-hydroxylase expression by lipopolysaccharide. J. Lipids Res. 2009. 50: 2258-2264.}},
author = {{Diczfalusy, Ulf and Berg, Katarina and Carlsson, Ann-Margreth and Gong, Mei and Golenbock, Douglas T. and Rooyackers, Olav and Flaring, Urban and Björkbacka, Harry}},
issn = {{1539-7262}},
keywords = {{sepsis; inflammation; endotoxin; 25-hydroxycholesterol; oxysterols; macrophages}},
language = {{eng}},
number = {{11}},
pages = {{2258--2264}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Lipid Research}},
title = {{Marked upregulation of cholesterol 25-hydroxylase expression by lipopolysaccharide}},
url = {{http://dx.doi.org/10.1194/jlr.M900107-JLR200}},
doi = {{10.1194/jlr.M900107-JLR200}},
volume = {{50}},
year = {{2009}},
}