Bi-enzyme alcohol biosensors based on genetically engineered alcohol oxidase and different peroxidases
(2005) In Microchimica Acta 152(1-2). p.21-27- Abstract
- We report on the development of a bi-layer bi-enzyme biosensor architecture using different peroxidases and alcohol oxidase from Hansenula polymorpha C-105 as biological recognition elements. The sensor architecture comprises a first layer containing either horseradish peroxidase or royal palm tree peroxidase crosslinked with an Osmium complex-modified redox hydrogel. On top, a second layer was formed by electrochemically induced precipitation of a cathodic electrodeposition paint simultaneously entrapping alcohol oxidase isolated from a genetically modified strain of Hansenula polymorpha C-105. The sensor architecture was optimized with respect to effective electron transfer and stability of the enzyme. The main characteristics of the... (More)
- We report on the development of a bi-layer bi-enzyme biosensor architecture using different peroxidases and alcohol oxidase from Hansenula polymorpha C-105 as biological recognition elements. The sensor architecture comprises a first layer containing either horseradish peroxidase or royal palm tree peroxidase crosslinked with an Osmium complex-modified redox hydrogel. On top, a second layer was formed by electrochemically induced precipitation of a cathodic electrodeposition paint simultaneously entrapping alcohol oxidase isolated from a genetically modified strain of Hansenula polymorpha C-105. The sensor architecture was optimized with respect to effective electron transfer and stability of the enzyme. The main characteristics of the biosensors are an apparent maximal current I-max(app) of 572-940 nA, an apparent Michaelis constant K-M(app) of 9.5 mM, a sensitivity of 60-98 nA mM(-1) and an improved operational stability represented by a deactivation constant of 1.5-2.0 x 10(-4) min(-1). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150872
- author
- Alpeeva, IS ; Vilkanauskyte, A ; Ngounou, B ; Csöregi, Elisabeth LU ; Sakharov, IY ; Gonchar, M and Schuhmann, W
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microchimica Acta
- volume
- 152
- issue
- 1-2
- pages
- 21 - 27
- publisher
- Springer
- external identifiers
-
- wos:000233235700002
- scopus:27744591593
- ISSN
- 1436-5073
- DOI
- 10.1007/s00604-005-0407-7
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- ff77032e-cd9f-4fb3-be71-7db6bf0e4f07 (old id 150872)
- date added to LUP
- 2016-04-01 12:12:43
- date last changed
- 2023-01-03 05:22:45
@article{ff77032e-cd9f-4fb3-be71-7db6bf0e4f07,
abstract = {{We report on the development of a bi-layer bi-enzyme biosensor architecture using different peroxidases and alcohol oxidase from Hansenula polymorpha C-105 as biological recognition elements. The sensor architecture comprises a first layer containing either horseradish peroxidase or royal palm tree peroxidase crosslinked with an Osmium complex-modified redox hydrogel. On top, a second layer was formed by electrochemically induced precipitation of a cathodic electrodeposition paint simultaneously entrapping alcohol oxidase isolated from a genetically modified strain of Hansenula polymorpha C-105. The sensor architecture was optimized with respect to effective electron transfer and stability of the enzyme. The main characteristics of the biosensors are an apparent maximal current I-max(app) of 572-940 nA, an apparent Michaelis constant K-M(app) of 9.5 mM, a sensitivity of 60-98 nA mM(-1) and an improved operational stability represented by a deactivation constant of 1.5-2.0 x 10(-4) min(-1).}},
author = {{Alpeeva, IS and Vilkanauskyte, A and Ngounou, B and Csöregi, Elisabeth and Sakharov, IY and Gonchar, M and Schuhmann, W}},
issn = {{1436-5073}},
language = {{eng}},
number = {{1-2}},
pages = {{21--27}},
publisher = {{Springer}},
series = {{Microchimica Acta}},
title = {{Bi-enzyme alcohol biosensors based on genetically engineered alcohol oxidase and different peroxidases}},
url = {{http://dx.doi.org/10.1007/s00604-005-0407-7}},
doi = {{10.1007/s00604-005-0407-7}},
volume = {{152}},
year = {{2005}},
}