Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin beta-ODAP (beta-N-oxalyl-L-alpha,beta-diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system
(2005) In Journal of the Science of Food and Agriculture 85(12). p.2027-2032- Abstract
- Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and... (More)
- Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and horseradish peroxidase (HRP), on the end of a spectrographic graphite electrode. beta-ODAP is oxidised by GlOx to form H2O2, which in turn is bioelectrocatalytically reduced by HRP through a mediated reaction using a polymeric mediator incorporating Os2+/3+ functionalities rapidly shuttling electrons with the electrode-giving rise to the analytical signal. On the basis of this analysis system, the new isolates are capable of utilising beta-ODAP as sole carbon source to a maximum of 90-95% within 5 days with concomitant increase in cell protein. (c) 2005 Society of Chemical Industry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150974
- author
- Marichamy, Sankar LU ; Yigzaw, Yirgalem LU ; Gorton, Lo LU and Mattiasson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the Science of Food and Agriculture
- volume
- 85
- issue
- 12
- pages
- 2027 - 2032
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000231633800010
- scopus:24344495816
- ISSN
- 1097-0010
- DOI
- 10.1002/jsfa.2211
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biotechnology (LTH) (011001037), Analytical Chemistry (S/LTH) (011001004)
- id
- e42e61d9-50a3-43a4-af35-dcb90ba523f5 (old id 150974)
- date added to LUP
- 2016-04-01 16:07:54
- date last changed
- 2022-01-28 17:28:15
@article{e42e61d9-50a3-43a4-af35-dcb90ba523f5, abstract = {{Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and horseradish peroxidase (HRP), on the end of a spectrographic graphite electrode. beta-ODAP is oxidised by GlOx to form H2O2, which in turn is bioelectrocatalytically reduced by HRP through a mediated reaction using a polymeric mediator incorporating Os2+/3+ functionalities rapidly shuttling electrons with the electrode-giving rise to the analytical signal. On the basis of this analysis system, the new isolates are capable of utilising beta-ODAP as sole carbon source to a maximum of 90-95% within 5 days with concomitant increase in cell protein. (c) 2005 Society of Chemical Industry.}}, author = {{Marichamy, Sankar and Yigzaw, Yirgalem and Gorton, Lo and Mattiasson, Bo}}, issn = {{1097-0010}}, language = {{eng}}, number = {{12}}, pages = {{2027--2032}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of the Science of Food and Agriculture}}, title = {{Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin beta-ODAP (beta-N-oxalyl-L-alpha,beta-diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system}}, url = {{http://dx.doi.org/10.1002/jsfa.2211}}, doi = {{10.1002/jsfa.2211}}, volume = {{85}}, year = {{2005}}, }