Binding of charged ligands to macromolecules. Anomalous salt dependence
(2005) In The Journal of Physical Chemistry Part B 109(5). p.2007-2013- Abstract
- Although interactions in biological systems occur in the presence of a large number of charged species, the binding of charged ligands to different biomolecules is often analyzed in a simplified model focusing only upon the receptor, ligand, and added salt. Here we demonstrate that the presence of charged macromolecules can affect binding to the receptor in an unexpected way. Experimental studies of the binding of barium ions to the chelator5,5'-dibromo-1,2-bis(O-amino-phenoxy)-ethane-N,N,N',N'-tetraacetic acid in the presence of charged silica sols show that the binding affinity increases with added salt. The experimental findings are verified in Monte Carlo simulations using a dielectric continuum model with a uniform dielectric... (More)
- Although interactions in biological systems occur in the presence of a large number of charged species, the binding of charged ligands to different biomolecules is often analyzed in a simplified model focusing only upon the receptor, ligand, and added salt. Here we demonstrate that the presence of charged macromolecules can affect binding to the receptor in an unexpected way. Experimental studies of the binding of barium ions to the chelator5,5'-dibromo-1,2-bis(O-amino-phenoxy)-ethane-N,N,N',N'-tetraacetic acid in the presence of charged silica sols show that the binding affinity increases with added salt. The experimental findings are verified in Monte Carlo simulations using a dielectric continuum model with a uniform dielectric permittivity throughout the solution. The anomalous salt behavior is caused by a reduction of the chemical potential of the free ligand, which even in the absence of binding interacts strongly with the oppositely charged receptor. These results are also relevant for the interpretation of competition studies often used in the case of strong ligand binding. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151561
- author
- da Silva, Fernando Luis B ; Linse, Sara LU and Jönsson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part B
- volume
- 109
- issue
- 5
- pages
- 2007 - 2013
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000226779900051
- scopus:13444257969
- pmid:16851185
- ISSN
- 1520-5207
- DOI
- 10.1021/jp049304o
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039), Biophysical Chemistry (LTH) (011001011)
- id
- 10dca575-57af-424f-8ae6-25f5dd5373c9 (old id 151561)
- date added to LUP
- 2016-04-01 16:43:59
- date last changed
- 2023-01-05 01:50:27
@article{10dca575-57af-424f-8ae6-25f5dd5373c9,
abstract = {{Although interactions in biological systems occur in the presence of a large number of charged species, the binding of charged ligands to different biomolecules is often analyzed in a simplified model focusing only upon the receptor, ligand, and added salt. Here we demonstrate that the presence of charged macromolecules can affect binding to the receptor in an unexpected way. Experimental studies of the binding of barium ions to the chelator5,5'-dibromo-1,2-bis(O-amino-phenoxy)-ethane-N,N,N',N'-tetraacetic acid in the presence of charged silica sols show that the binding affinity increases with added salt. The experimental findings are verified in Monte Carlo simulations using a dielectric continuum model with a uniform dielectric permittivity throughout the solution. The anomalous salt behavior is caused by a reduction of the chemical potential of the free ligand, which even in the absence of binding interacts strongly with the oppositely charged receptor. These results are also relevant for the interpretation of competition studies often used in the case of strong ligand binding.}},
author = {{da Silva, Fernando Luis B and Linse, Sara and Jönsson, Bo}},
issn = {{1520-5207}},
language = {{eng}},
number = {{5}},
pages = {{2007--2013}},
publisher = {{The American Chemical Society (ACS)}},
series = {{The Journal of Physical Chemistry Part B}},
title = {{Binding of charged ligands to macromolecules. Anomalous salt dependence}},
url = {{http://dx.doi.org/10.1021/jp049304o}},
doi = {{10.1021/jp049304o}},
volume = {{109}},
year = {{2005}},
}