Enhanced protein adsorption due to charge regulation
(2005) In Langmuir 21(18). p.8385-8388- Abstract
- When a protein molecule approaches a charged surface, its protonation state can undergo dramatic changes due to the imposed electric potential. This has a large impact on adsorption strengths that may be enhanced by several kT. Using mesoscopic simulation techniques as well as analytical theories, we have investigated this regulation mechanism and demonstrate how it is influenced by salt concentration and solution pH. Using hisactophilin as a test case, we show how the binding to a lipid membrane is governed by small changes in pH and that this is intimately coupled to the charge regulation mechanism.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/152706
- author
- Lund, Mikael LU ; Åkesson, Torbjörn LU and Jönsson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 21
- issue
- 18
- pages
- 8385 - 8388
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000231550000044
- scopus:24944550836
- ISSN
- 0743-7463
- DOI
- 10.1021/la050607z
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039)
- id
- b9508064-e415-49da-bcdc-7e04a5be6620 (old id 152706)
- date added to LUP
- 2016-04-01 11:51:01
- date last changed
- 2023-01-03 00:12:01
@article{b9508064-e415-49da-bcdc-7e04a5be6620, abstract = {{When a protein molecule approaches a charged surface, its protonation state can undergo dramatic changes due to the imposed electric potential. This has a large impact on adsorption strengths that may be enhanced by several kT. Using mesoscopic simulation techniques as well as analytical theories, we have investigated this regulation mechanism and demonstrate how it is influenced by salt concentration and solution pH. Using hisactophilin as a test case, we show how the binding to a lipid membrane is governed by small changes in pH and that this is intimately coupled to the charge regulation mechanism.}}, author = {{Lund, Mikael and Åkesson, Torbjörn and Jönsson, Bo}}, issn = {{0743-7463}}, language = {{eng}}, number = {{18}}, pages = {{8385--8388}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Langmuir}}, title = {{Enhanced protein adsorption due to charge regulation}}, url = {{http://dx.doi.org/10.1021/la050607z}}, doi = {{10.1021/la050607z}}, volume = {{21}}, year = {{2005}}, }