Enzymatic degradation of model cellulose films
(2005) In Journal of Colloid and Interface Science 284(1). p.99-106- Abstract
- Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial... (More)
- Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial film. At long degradation times, however, there was an expansion of the cellulose film. The enzyme concentration affected the degradation rate significantly, with a faster degradation at a higher enzyme concentration. The effects of pH, temperature, ionic strength and stirring rate in the cuvette were also investigated. (c) 2004 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/157364
- author
- Eriksson, Jonny ; Malmsten, M ; Tiberg, Fredrik LU ; Callisen, T H ; Damhus, T and Johansen, K S
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Colloid and Interface Science
- volume
- 284
- issue
- 1
- pages
- 99 - 106
- publisher
- Elsevier
- external identifiers
-
- pmid:15752790
- wos:000227819700014
- scopus:14844303745
- pmid:15752790
- ISSN
- 1095-7103
- DOI
- 10.1016/j.jcis.2004.10.041
- language
- English
- LU publication?
- yes
- id
- d9101812-33fa-40c4-becf-13f1ae8290a6 (old id 157364)
- date added to LUP
- 2016-04-01 11:43:54
- date last changed
- 2022-01-26 17:23:16
@article{d9101812-33fa-40c4-becf-13f1ae8290a6,
abstract = {{Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial film. At long degradation times, however, there was an expansion of the cellulose film. The enzyme concentration affected the degradation rate significantly, with a faster degradation at a higher enzyme concentration. The effects of pH, temperature, ionic strength and stirring rate in the cuvette were also investigated. (c) 2004 Elsevier Inc. All rights reserved.}},
author = {{Eriksson, Jonny and Malmsten, M and Tiberg, Fredrik and Callisen, T H and Damhus, T and Johansen, K S}},
issn = {{1095-7103}},
language = {{eng}},
number = {{1}},
pages = {{99--106}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Enzymatic degradation of model cellulose films}},
url = {{http://dx.doi.org/10.1016/j.jcis.2004.10.041}},
doi = {{10.1016/j.jcis.2004.10.041}},
volume = {{284}},
year = {{2005}},
}