Composition of supported model membranes determined by neutron reflection
(2005) In Langmuir 21(7). p.2827-2837- Abstract
- We have investigated the formation of supported bilayers by coadsorption of dipalmitoyl phosphatidylcholine (DPPC) with the nonionic surfactant P-D-dodecyl maltoside. The adsorption of mixed phospholipid-surfactant micelles on hydrophilic silica surfaces at 25 degrees C was followed as a function of bulk concentration by neutron reflection. Using chain-deuterated d(25)-beta-D-dodecyl maltoside and d(62)-DPPC, we demonstrate that it is possible to determine the composition of the bilayers at each stage of a sequential dilution process, which enriches the adsorbed layer in phospholipid and leads to complete elimination of the surfactant. The final supported bilayers have thicknesses of 51 +/- 3 angstrom and are stable to heating to 37... (More)
- We have investigated the formation of supported bilayers by coadsorption of dipalmitoyl phosphatidylcholine (DPPC) with the nonionic surfactant P-D-dodecyl maltoside. The adsorption of mixed phospholipid-surfactant micelles on hydrophilic silica surfaces at 25 degrees C was followed as a function of bulk concentration by neutron reflection. Using chain-deuterated d(25)-beta-D-dodecyl maltoside and d(62)-DPPC, we demonstrate that it is possible to determine the composition of the bilayers at each stage of a sequential dilution process, which enriches the adsorbed layer in phospholipid and leads to complete elimination of the surfactant. The final supported bilayers have thicknesses of 51 +/- 3 angstrom and are stable to heating to 37 degrees C once all surfactant has been removed, and the structures agree well with other published data on DPPC supported bilayers. The coadsorption of cholesterol in a DPPC-surfactant mixture was also achieved, and the location and volume fraction of cholesterol in the DPPC bilayer was determined. Cholesterol is located in a 18 +/- 1 angstrom thick layer below the lipid headgroup region and leads to an increased bilayer thickness of 58 +/- 2 angstrom at 26 mol % of cholesterol. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/157642
- author
- Vacklin, H P ; Tiberg, Fredrik LU ; Fragneto, G and Thomas, R K
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 21
- issue
- 7
- pages
- 2827 - 2837
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:16244379573
- ISSN
- 0743-7463
- DOI
- 10.1021/la047389e
- language
- English
- LU publication?
- yes
- id
- 60a8c3c8-4778-4983-b717-2573bf164d03 (old id 157642)
- date added to LUP
- 2016-04-01 11:44:40
- date last changed
- 2022-03-20 18:11:47
@article{60a8c3c8-4778-4983-b717-2573bf164d03,
abstract = {{We have investigated the formation of supported bilayers by coadsorption of dipalmitoyl phosphatidylcholine (DPPC) with the nonionic surfactant P-D-dodecyl maltoside. The adsorption of mixed phospholipid-surfactant micelles on hydrophilic silica surfaces at 25 degrees C was followed as a function of bulk concentration by neutron reflection. Using chain-deuterated d(25)-beta-D-dodecyl maltoside and d(62)-DPPC, we demonstrate that it is possible to determine the composition of the bilayers at each stage of a sequential dilution process, which enriches the adsorbed layer in phospholipid and leads to complete elimination of the surfactant. The final supported bilayers have thicknesses of 51 +/- 3 angstrom and are stable to heating to 37 degrees C once all surfactant has been removed, and the structures agree well with other published data on DPPC supported bilayers. The coadsorption of cholesterol in a DPPC-surfactant mixture was also achieved, and the location and volume fraction of cholesterol in the DPPC bilayer was determined. Cholesterol is located in a 18 +/- 1 angstrom thick layer below the lipid headgroup region and leads to an increased bilayer thickness of 58 +/- 2 angstrom at 26 mol % of cholesterol.}},
author = {{Vacklin, H P and Tiberg, Fredrik and Fragneto, G and Thomas, R K}},
issn = {{0743-7463}},
language = {{eng}},
number = {{7}},
pages = {{2827--2837}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Langmuir}},
title = {{Composition of supported model membranes determined by neutron reflection}},
url = {{http://dx.doi.org/10.1021/la047389e}},
doi = {{10.1021/la047389e}},
volume = {{21}},
year = {{2005}},
}