Retinal progenitor cell xenografts to the pig retina: immunological reactions.
Warfvinge, Karin LU
; Kiilgaard, Jens F
; Klassen, Henry
; Zamiri, Parisa
; Scherfig, Erik
; Streilein, Wayne
; Prause, Jan U
and Young, Michael J
(2006)
In Cell Transplantation
15(7).
p.603-612
- Abstract
- We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and... (More)
- We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2-5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/163930
- author
- Warfvinge, Karin
LU
; Kiilgaard, Jens F
; Klassen, Henry
; Zamiri, Parisa
; Scherfig, Erik
; Streilein, Wayne
; Prause, Jan U
and Young, Michael J
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- stem cell, xenografts, transplant, retina
- in
- Cell Transplantation
- volume
- 15
- issue
- 7
- pages
- 603 - 612
- publisher
- Cognizant Communication Corporation
- external identifiers
-
- wos:000242553500004
- scopus:33845418755
- ISSN
- 1555-3892
- language
- English
- LU publication?
- yes
- id
- 165258a1-beb3-46fd-9867-1f3a5f9edcce (old id 163930)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17176612&dopt=Abstract
- date added to LUP
- 2016-04-01 11:53:23
- date last changed
- 2022-02-10 22:58:29
@article{165258a1-beb3-46fd-9867-1f3a5f9edcce,
abstract = {{We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2-5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection.}},
author = {{Warfvinge, Karin and Kiilgaard, Jens F and Klassen, Henry and Zamiri, Parisa and Scherfig, Erik and Streilein, Wayne and Prause, Jan U and Young, Michael J}},
issn = {{1555-3892}},
keywords = {{stem cell; xenografts; transplant; retina}},
language = {{eng}},
number = {{7}},
pages = {{603--612}},
publisher = {{Cognizant Communication Corporation}},
series = {{Cell Transplantation}},
title = {{Retinal progenitor cell xenografts to the pig retina: immunological reactions.}},
url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17176612&dopt=Abstract}},
volume = {{15}},
year = {{2006}},
}