N-acetylation of arylalkylmines (serotonin and tryptamine) in the crayfish brain.
(1989) In Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 93. p.307-312- Abstract
- 1. The presence of N-acetyltransferase in the crayfish brain (Pacifastacus leniusculus) was investigated. The arylalkylamines tryptamine and 14C-serotonin, are acetylated by crayfish brain homogenates with the acetyl donors 3H-acetyl coenzyme A and N, S-diacetylcysteamine, respectively.
2. Resolution of serotonin metabolites by thin layer chromatography (chloroform/methanol/glacial acetic acid, 93 + 7 + 1 and ethyl acetate) revealed only one radioactive product (N-acetylserotonin, Rf 0.31) after enzymatic reaction. For example, melatonin and 5-hydroxyindole acetic acid could not be detected.
3. This was also confirmed by reversed phase high performance liquid chromatography indicating the absence of monoamine oxidase and... (More) - 1. The presence of N-acetyltransferase in the crayfish brain (Pacifastacus leniusculus) was investigated. The arylalkylamines tryptamine and 14C-serotonin, are acetylated by crayfish brain homogenates with the acetyl donors 3H-acetyl coenzyme A and N, S-diacetylcysteamine, respectively.
2. Resolution of serotonin metabolites by thin layer chromatography (chloroform/methanol/glacial acetic acid, 93 + 7 + 1 and ethyl acetate) revealed only one radioactive product (N-acetylserotonin, Rf 0.31) after enzymatic reaction. For example, melatonin and 5-hydroxyindole acetic acid could not be detected.
3. This was also confirmed by reversed phase high performance liquid chromatography indicating the absence of monoamine oxidase and hydroxyindole-O-methyltransferase activity under the experimental conditions used.
4. Inhibitory studies with cystamine suggest the involvement of heat sensitive disulfide compounds in the regulation of crayfish brain-N-acetyltransferase.
5. N-acetylation seems to be a major pathway in the metabolism of biogenic amines in the crayfish central nervous system. (Less)
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https://lup.lub.lu.se/record/176da897-f7ea-466a-bc82-be05260d4bc6
- author
- Dubbels, R and Elofsson, Rolf LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
- volume
- 93
- pages
- 307 - 312
- publisher
- Elsevier
- external identifiers
-
- scopus:0024458159
- ISSN
- 1532-0456
- DOI
- 10.1016/0742-8413(89)90238-7
- language
- English
- LU publication?
- yes
- id
- 176da897-f7ea-466a-bc82-be05260d4bc6
- date added to LUP
- 2016-11-07 18:42:04
- date last changed
- 2021-01-03 04:54:40
@article{176da897-f7ea-466a-bc82-be05260d4bc6,
abstract = {{1. The presence of N-acetyltransferase in the crayfish brain (Pacifastacus leniusculus) was investigated. The arylalkylamines tryptamine and 14C-serotonin, are acetylated by crayfish brain homogenates with the acetyl donors 3H-acetyl coenzyme A and N, S-diacetylcysteamine, respectively.<br/><br/>2. Resolution of serotonin metabolites by thin layer chromatography (chloroform/methanol/glacial acetic acid, 93 + 7 + 1 and ethyl acetate) revealed only one radioactive product (N-acetylserotonin, Rf 0.31) after enzymatic reaction. For example, melatonin and 5-hydroxyindole acetic acid could not be detected.<br/><br/>3. This was also confirmed by reversed phase high performance liquid chromatography indicating the absence of monoamine oxidase and hydroxyindole-O-methyltransferase activity under the experimental conditions used.<br/><br/>4. Inhibitory studies with cystamine suggest the involvement of heat sensitive disulfide compounds in the regulation of crayfish brain-N-acetyltransferase.<br/><br/>5. N-acetylation seems to be a major pathway in the metabolism of biogenic amines in the crayfish central nervous system.}},
author = {{Dubbels, R and Elofsson, Rolf}},
issn = {{1532-0456}},
language = {{eng}},
pages = {{307--312}},
publisher = {{Elsevier}},
series = {{Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology}},
title = {{N-acetylation of arylalkylmines (serotonin and tryptamine) in the crayfish brain.}},
url = {{http://dx.doi.org/10.1016/0742-8413(89)90238-7}},
doi = {{10.1016/0742-8413(89)90238-7}},
volume = {{93}},
year = {{1989}},
}