Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

An Amperometric Lactate Sensor Based on a NAD+-Analog and Lactate Dehydrogenase Coimmobilized on Reticulated Vitreous Carbon

Khayyami, Masoud ; Peña Garcia, Nuria ; Larsson, Per-Olof LU ; Danielsson, Bengt LU and Johansson, Gillis (1997) In Electroanalysis 9(7). p.523-526
Abstract
A NAD+-analog was coimmobilized with lactate dehydrogenase
(LDH) on reticulated vitreous carbon (RVC) to give an amperometric
lactate biosensor. Both LDH and the NAD+ -analog were bound
covalently with carbodiimide to the surface of the porous RVC-material.
The electrode was operated in a FIA-arrangement in the presence or
absence of a soluble mediator. Meldola Blue. The stability was poor when
the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of
mediator but improved most significantly in the presence of 5 μM
mediator, so that 65% of the original activity remained after 16 days.
The amperometric currents were smaller with regeneration by mediator at
... (More)
A NAD+-analog was coimmobilized with lactate dehydrogenase
(LDH) on reticulated vitreous carbon (RVC) to give an amperometric
lactate biosensor. Both LDH and the NAD+ -analog were bound
covalently with carbodiimide to the surface of the porous RVC-material.
The electrode was operated in a FIA-arrangement in the presence or
absence of a soluble mediator. Meldola Blue. The stability was poor when
the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of
mediator but improved most significantly in the presence of 5 μM
mediator, so that 65% of the original activity remained after 16 days.
The amperometric currents were smaller with regeneration by mediator at
−100 mV than with direct electrochemical oxidation at +400 mV,
indicating that the additional steps slow down the reaction rate. Linear
calibration plots were obtained from the detection limit, 1 μM, to 500
μM lactate with 5 μM mediator, reoxidized at −100 mV. The sample
throughput was about 60 h−1. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electroanalysis
volume
9
issue
7
pages
4 pages
publisher
Wiley-VCH Verlag
external identifiers
  • scopus:0000183811
ISSN
1040-0397
DOI
10.1002/elan.1140090703
language
English
LU publication?
yes
id
17a7b3cc-8fb5-4c45-b576-3ac6ddd95bd6
date added to LUP
2024-06-17 10:30:14
date last changed
2025-10-14 09:29:42
@article{17a7b3cc-8fb5-4c45-b576-3ac6ddd95bd6,
  abstract     = {{A NAD<sup>+</sup>-analog was coimmobilized with lactate dehydrogenase <br>
(LDH) on reticulated vitreous carbon (RVC) to give an amperometric <br>
lactate biosensor. Both LDH and the NAD<sup>+</sup> -analog were bound <br>
covalently with carbodiimide to the surface of the porous RVC-material. <br>
The electrode was operated in a FIA-arrangement in the presence or <br>
absence of a soluble mediator. Meldola Blue. The stability was poor when<br>
 the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of <br>
mediator but improved most significantly in the presence of 5 μM <br>
mediator, so that 65% of the original activity remained after 16 days. <br>
The amperometric currents were smaller with regeneration by mediator at <br>
−100 mV than with direct electrochemical oxidation at +400 mV, <br>
indicating that the additional steps slow down the reaction rate. Linear<br>
 calibration plots were obtained from the detection limit, 1 μM, to 500 <br>
μM lactate with 5 μM mediator, reoxidized at −100 mV. The sample <br>
throughput was about 60 h<sup>−1</sup>.}},
  author       = {{Khayyami, Masoud and Peña Garcia, Nuria and Larsson, Per-Olof and Danielsson, Bengt and Johansson, Gillis}},
  issn         = {{1040-0397}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{523--526}},
  publisher    = {{Wiley-VCH Verlag}},
  series       = {{Electroanalysis}},
  title        = {{An Amperometric Lactate Sensor Based on a NAD<sup>+</sup>-Analog and Lactate Dehydrogenase Coimmobilized on Reticulated Vitreous Carbon}},
  url          = {{http://dx.doi.org/10.1002/elan.1140090703}},
  doi          = {{10.1002/elan.1140090703}},
  volume       = {{9}},
  year         = {{1997}},
}