Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties
(2011) In Molecular Membrane Biology 28(1). p.42-53- Abstract
- Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the... (More)
- Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the physical properties of formulations with and without ALA were characterized using cryo-transmission electron microscopy, dynamic light scattering, and an ethidium bromide intercalation assay. ALA seems to have limited influence on the initial internal structure of the complexes and the protection of DNA, but its presence is found to decrease the average effective size of the dispersed particles; this change in size may be important in improving the biological activity. Furthermore, ALA can act to influence the transfection efficiency of the formulation by promoting the release of DNA following internalization in the transfected cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1877898
- author
- Lundberg, Dan LU ; Faneca, Henrique ; Moran, Maria Del Carmen ; Pedroso De Lima, Maria C. ; Miguel, Maria Da Graca and Lindman, Björn LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gene therapy, colloid science, mechanism
- in
- Molecular Membrane Biology
- volume
- 28
- issue
- 1
- pages
- 42 - 53
- publisher
- Informa Healthcare
- external identifiers
-
- wos:000287154300004
- scopus:78651279670
- pmid:21219253
- ISSN
- 0968-7688
- DOI
- 10.3109/09687688.2010.508879
- language
- English
- LU publication?
- yes
- id
- 186f74f2-a48c-4e0b-aef9-d7a263bb890c (old id 1877898)
- date added to LUP
- 2016-04-01 15:01:49
- date last changed
- 2022-01-28 03:43:09
@article{186f74f2-a48c-4e0b-aef9-d7a263bb890c,
abstract = {{Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the physical properties of formulations with and without ALA were characterized using cryo-transmission electron microscopy, dynamic light scattering, and an ethidium bromide intercalation assay. ALA seems to have limited influence on the initial internal structure of the complexes and the protection of DNA, but its presence is found to decrease the average effective size of the dispersed particles; this change in size may be important in improving the biological activity. Furthermore, ALA can act to influence the transfection efficiency of the formulation by promoting the release of DNA following internalization in the transfected cells.}},
author = {{Lundberg, Dan and Faneca, Henrique and Moran, Maria Del Carmen and Pedroso De Lima, Maria C. and Miguel, Maria Da Graca and Lindman, Björn}},
issn = {{0968-7688}},
keywords = {{Gene therapy; colloid science; mechanism}},
language = {{eng}},
number = {{1}},
pages = {{42--53}},
publisher = {{Informa Healthcare}},
series = {{Molecular Membrane Biology}},
title = {{Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties}},
url = {{http://dx.doi.org/10.3109/09687688.2010.508879}},
doi = {{10.3109/09687688.2010.508879}},
volume = {{28}},
year = {{2011}},
}