DNA movement in sub-20 nm nanoslits
(2007) 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 In Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 p.1201-1203- Abstract
The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of... (More)
The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.
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- author
- Salieb-Beugelaar, Georgette ; Teapal, Juliane ; van Nieuwkasteele, Jan ; Wijnperlé, Daniël ; Tegenfeldt, Jonas O. LU ; Eijkel, Jan C.T. and van den Berg, Albert
- organization
- publishing date
- 2007
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA, Intermittent movement, Nanoslits
- host publication
- Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
- series title
- Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
- editor
- Viovy, Jean-Louis ; Tabeling, Patrick ; Descroix, Stephanie and Malaquin, Laurent
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
- conference location
- Paris, France
- conference dates
- 2007-10-07 - 2007-10-11
- external identifiers
-
- scopus:57349152760
- ISBN
- 9780979806407
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2007 CBMS.
- id
- 18f1821b-ed0d-469d-9b5c-09b388b49b9b
- date added to LUP
- 2022-11-10 14:15:57
- date last changed
- 2023-11-12 07:50:18
@inproceedings{18f1821b-ed0d-469d-9b5c-09b388b49b9b, abstract = {{<p>The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.</p>}}, author = {{Salieb-Beugelaar, Georgette and Teapal, Juliane and van Nieuwkasteele, Jan and Wijnperlé, Daniël and Tegenfeldt, Jonas O. and Eijkel, Jan C.T. and van den Berg, Albert}}, booktitle = {{Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007}}, editor = {{Viovy, Jean-Louis and Tabeling, Patrick and Descroix, Stephanie and Malaquin, Laurent}}, isbn = {{9780979806407}}, keywords = {{DNA; Intermittent movement; Nanoslits}}, language = {{eng}}, pages = {{1201--1203}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007}}, title = {{DNA movement in sub-20 nm nanoslits}}, year = {{2007}}, }