Development of amperometric immunoassays (AIAs) for calpastatin and mu-calpain with possible applications in the biomedical field
(2011) In Sensors and Actuators B: Chemical 152(2). p.248-253- Abstract
- Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and... (More)
- Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and possibility for miniaturization. (C) 2011 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1918836
- author
- Zor, Kinga LU ; Zabara, Alexandru LU ; Gorton, Lo LU and Nistor, Mihaela LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amperometric immunoassay, Calpastatin, Calpain, Pathology
- in
- Sensors and Actuators B: Chemical
- volume
- 152
- issue
- 2
- pages
- 248 - 253
- publisher
- Elsevier
- external identifiers
-
- wos:000288877700016
- scopus:79952442892
- ISSN
- 0925-4005
- DOI
- 10.1016/j.snb.2010.12.016
- language
- English
- LU publication?
- yes
- id
- 75d7844d-02aa-4a67-a0d9-30397e272e09 (old id 1918836)
- date added to LUP
- 2016-04-01 14:10:33
- date last changed
- 2022-01-27 23:12:17
@article{75d7844d-02aa-4a67-a0d9-30397e272e09,
abstract = {{Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and possibility for miniaturization. (C) 2011 Elsevier B.V. All rights reserved.}},
author = {{Zor, Kinga and Zabara, Alexandru and Gorton, Lo and Nistor, Mihaela}},
issn = {{0925-4005}},
keywords = {{Amperometric immunoassay; Calpastatin; Calpain; Pathology}},
language = {{eng}},
number = {{2}},
pages = {{248--253}},
publisher = {{Elsevier}},
series = {{Sensors and Actuators B: Chemical}},
title = {{Development of amperometric immunoassays (AIAs) for calpastatin and mu-calpain with possible applications in the biomedical field}},
url = {{http://dx.doi.org/10.1016/j.snb.2010.12.016}},
doi = {{10.1016/j.snb.2010.12.016}},
volume = {{152}},
year = {{2011}},
}