A new murine IgG1 anti-Tn monoclonal antibody with in vivo anti tumour activity.
(2011) In Glycobiology 21(8). p.1097-1107- Abstract
- The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and... (More)
- The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and pancreas cancer. The GOD3-2C4 antibody has ADCC activity against Jurkat cells in vitro and for the first time it can be shown that an anti-Tn antibody has a significant in vivo effect on a human cancer cell line grown as a xenograft in SCID mice. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937400
- author
- Welinder, Charlotte LU ; Baldetorp, Bo LU ; Borrebaeck, Carl ; Fredlund, Britt-Marie and Jansson, Bo LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- monoclonal antibody, in vivo therapy, antigen, Tn, anti-carbohydrate, biomarker
- in
- Glycobiology
- volume
- 21
- issue
- 8
- pages
- 1097 - 1107
- publisher
- Oxford University Press
- external identifiers
-
- wos:000292557600012
- pmid:21470982
- scopus:79960275033
- pmid:21470982
- ISSN
- 1460-2423
- DOI
- 10.1093/glycob/cwr048
- language
- English
- LU publication?
- yes
- id
- 7101089a-2165-40e6-bc7a-e5d5e3a9d4b2 (old id 1937400)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21470982?dopt=Abstract
- date added to LUP
- 2016-04-01 14:49:17
- date last changed
- 2022-03-14 07:54:54
@article{7101089a-2165-40e6-bc7a-e5d5e3a9d4b2, abstract = {{The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and pancreas cancer. The GOD3-2C4 antibody has ADCC activity against Jurkat cells in vitro and for the first time it can be shown that an anti-Tn antibody has a significant in vivo effect on a human cancer cell line grown as a xenograft in SCID mice.}}, author = {{Welinder, Charlotte and Baldetorp, Bo and Borrebaeck, Carl and Fredlund, Britt-Marie and Jansson, Bo}}, issn = {{1460-2423}}, keywords = {{monoclonal antibody; in vivo therapy; antigen; Tn; anti-carbohydrate; biomarker}}, language = {{eng}}, number = {{8}}, pages = {{1097--1107}}, publisher = {{Oxford University Press}}, series = {{Glycobiology}}, title = {{A new murine IgG1 anti-Tn monoclonal antibody with in vivo anti tumour activity.}}, url = {{http://dx.doi.org/10.1093/glycob/cwr048}}, doi = {{10.1093/glycob/cwr048}}, volume = {{21}}, year = {{2011}}, }