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CFEL TapeDrive 2.0: a conveyor belt-based sample-delivery system for multi-dimensional serial crystallography

Henkel, A. ; Maracke, J. ; Munke, A. LU orcid ; Galchenkova, M. ; Rahmani Mashhour, A. ; Reinke, P. ; Domaracky, M. ; Fleckenstein, H. ; Hakanpää, J. and Meyer, J. , et al. (2022) 33rd European Crystallographic Meeting, 2022 p.560-560
Abstract
Serial crystallography (SX) at both X-ray free-electron lasers (XFELs) and synchrotrons offers the possibility to collect data at non-cryogenic temperature almost radiation damage free and enables time-resolved crystallography of irreversible reactions. The necessary steady delivery of new micron-sized crystals of biological macromolecules sets it apart from traditional single crystal macromolecular crystallography (MX). For this, many new means of sample delivery have been developed [1]. Described here is a novel conveyor belt-based sample delivery system, the completely re-designed and reengineered second generation of the CFEL TapeDrive. It is optimized for fast installation at beamlines, ease of use, low sample consumption and precise... (More)
Serial crystallography (SX) at both X-ray free-electron lasers (XFELs) and synchrotrons offers the possibility to collect data at non-cryogenic temperature almost radiation damage free and enables time-resolved crystallography of irreversible reactions. The necessary steady delivery of new micron-sized crystals of biological macromolecules sets it apart from traditional single crystal macromolecular crystallography (MX). For this, many new means of sample delivery have been developed [1]. Described here is a novel conveyor belt-based sample delivery system, the completely re-designed and reengineered second generation of the CFEL TapeDrive. It is optimized for fast installation at beamlines, ease of use, low sample consumption and precise adjustment of several sample delivery parameters, like ligand concentration, pH and sample temperature. Through combination of these parameters and the additional possibility for time-resolved experiments (delay times of 50 ms – 180 s), CFEL TapeDrive 2.0 enables multidimensional serial crystallography experiments. It can now be used as standard instrumentation at beamline P11 at PETRA III (DESY, Germany) and is suitable for serial Laue crystallography with polychromatic X-rays. Additionally, it can be used at XFELs in air (e.g. at SwissFEL and LCLS), offering the possibility to combine the advantages of jet-based injection with those of fixed targetbased systems. In the future, parts of the system will be automated to achieve rapid ligand screening with micron-sized crystals in various sample environments. By introducing the CFEL TapeDrive 2.0, a platform for user-friendly multi-dimensional serial crystallography, we provide a novel approach for structural biology to further reveal details about how macromolecules keep our biological world turning. (Less)
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publishing date
type
Contribution to conference
publication status
published
subject
keywords
crystallography, X-ray crystallography, synchrotron, sample delivery, serial crystallography
pages
560 - 560
conference name
33rd European Crystallographic Meeting, 2022
conference location
Versailles, France
conference dates
2022-08-23 - 2022-08-27
DOI
10.1107/S2053273322092038
language
English
LU publication?
no
id
1964a292-af86-4574-b861-9b4c31f48992
date added to LUP
2025-04-21 21:18:04
date last changed
2025-04-29 11:32:52
@misc{1964a292-af86-4574-b861-9b4c31f48992,
  abstract     = {{Serial crystallography (SX) at both X-ray free-electron lasers (XFELs) and synchrotrons offers the possibility to collect data at non-cryogenic temperature almost radiation damage free and enables time-resolved crystallography of irreversible reactions. The necessary steady delivery of new micron-sized crystals of biological macromolecules sets it apart from traditional single crystal macromolecular crystallography (MX). For this, many new means of sample delivery have been developed [1]. Described here is a novel conveyor belt-based sample delivery system, the completely re-designed and reengineered second generation of the CFEL TapeDrive. It is optimized for fast installation at beamlines, ease of use, low sample consumption and precise adjustment of several sample delivery parameters, like ligand concentration, pH and sample temperature. Through combination of these parameters and the additional possibility for time-resolved experiments (delay times of 50 ms – 180 s), CFEL TapeDrive 2.0 enables multidimensional serial crystallography experiments. It can now be used as standard instrumentation at beamline P11 at PETRA III (DESY, Germany) and is suitable for serial Laue crystallography with polychromatic X-rays. Additionally, it can be used at XFELs in air (e.g. at SwissFEL and LCLS), offering the possibility to combine the advantages of jet-based injection with those of fixed targetbased systems. In the future, parts of the system will be automated to achieve rapid ligand screening with micron-sized crystals in various sample environments. By introducing the CFEL TapeDrive 2.0, a platform for user-friendly multi-dimensional serial crystallography, we provide a novel approach for structural biology to further reveal details about how macromolecules keep our biological world turning.}},
  author       = {{Henkel, A. and Maracke, J. and Munke, A. and Galchenkova, M. and Rahmani Mashhour, A. and Reinke, P. and Domaracky, M. and Fleckenstein, H. and Hakanpää, J. and Meyer, J. and Tolstikova, A. and Carnis, J. and Middendorf, P. and Gelisio, L. and Yefanov, O. and Chapman, H.N. and Oberthür, D.}},
  keywords     = {{crystallography; X-ray crystallography; synchrotron; sample delivery; serial crystallography}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{560--560}},
  title        = {{CFEL TapeDrive 2.0: a conveyor belt-based sample-delivery system for multi-dimensional serial crystallography}},
  url          = {{http://dx.doi.org/10.1107/S2053273322092038}},
  doi          = {{10.1107/S2053273322092038}},
  year         = {{2022}},
}