Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo
Madsen, O. D. ; Andersen, L. C. ; Michelsen, B. ; Owerbach, D. ; Larsson, L. I. ; Lernmark, A. LU
and Steiner, D. F.
(1988)
In Proceedings of the National Academy of Sciences of the United States of America
85(18).
p.6652-6656
- Abstract
The pluripotent rat islet tumor cell line MSL-G2 expresses primarily glucagon or cholecystokinin and not insulin in vitro but changes phenotype completely after prolonged in vivo cultivation to yield small-sized hypoglycemic tumors composed almost entirely of insulin-producing beta cells. When a genomic DNA fragment containing the coding and upstream regulatory regions of the human insulin gene was stably transfected into MSL-G2 cells no measurably amounts of insulin or insulin mRNA were detected in vitro. However, successive transplantation of two transfected clones resulted in hypoglycemic tumors that efficiently coexpressed human and rat insulin as determined by human C-peptide-specific immunoreagents. These results demonstrate that... (More)
The pluripotent rat islet tumor cell line MSL-G2 expresses primarily glucagon or cholecystokinin and not insulin in vitro but changes phenotype completely after prolonged in vivo cultivation to yield small-sized hypoglycemic tumors composed almost entirely of insulin-producing beta cells. When a genomic DNA fragment containing the coding and upstream regulatory regions of the human insulin gene was stably transfected into MSL-G2 cells no measurably amounts of insulin or insulin mRNA were detected in vitro. However, successive transplantation of two transfected clones resulted in hypoglycemic tumors that efficiently coexpressed human and rat insulin as determined by human C-peptide-specific immunoreagents. These results demonstrate that cis-acting tissue-specific insulin gene enhancer elements are conserved between rat and human insulin genes. We propose that the in vivo differentiation of MSL-G2 cells and transfected subclones into insulin-producing cells reflects processes of natural beta-cell ontogeny leading to insulin gene expression.
(Less)
- author
- Madsen, O. D.
; Andersen, L. C.
; Michelsen, B.
; Owerbach, D.
; Larsson, L. I.
; Lernmark, A.
LU
and Steiner, D. F.
- publishing date
- 1988-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 85
- issue
- 18
- pages
- 5 pages
- publisher
- National Academy of Sciences
- external identifiers
-
- pmid:2842785
- scopus:1542533888
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.85.18.6652
- language
- English
- LU publication?
- no
- id
- 19f79071-555c-4927-bec7-bb148af1bb0e
- date added to LUP
- 2019-09-11 10:01:37
- date last changed
- 2024-03-13 08:27:12
@article{19f79071-555c-4927-bec7-bb148af1bb0e,
abstract = {{<p>The pluripotent rat islet tumor cell line MSL-G2 expresses primarily glucagon or cholecystokinin and not insulin in vitro but changes phenotype completely after prolonged in vivo cultivation to yield small-sized hypoglycemic tumors composed almost entirely of insulin-producing beta cells. When a genomic DNA fragment containing the coding and upstream regulatory regions of the human insulin gene was stably transfected into MSL-G2 cells no measurably amounts of insulin or insulin mRNA were detected in vitro. However, successive transplantation of two transfected clones resulted in hypoglycemic tumors that efficiently coexpressed human and rat insulin as determined by human C-peptide-specific immunoreagents. These results demonstrate that cis-acting tissue-specific insulin gene enhancer elements are conserved between rat and human insulin genes. We propose that the in vivo differentiation of MSL-G2 cells and transfected subclones into insulin-producing cells reflects processes of natural beta-cell ontogeny leading to insulin gene expression.</p>}},
author = {{Madsen, O. D. and Andersen, L. C. and Michelsen, B. and Owerbach, D. and Larsson, L. I. and Lernmark, A. and Steiner, D. F.}},
issn = {{0027-8424}},
language = {{eng}},
month = {{01}},
number = {{18}},
pages = {{6652--6656}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences of the United States of America}},
title = {{Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo}},
url = {{http://dx.doi.org/10.1073/pnas.85.18.6652}},
doi = {{10.1073/pnas.85.18.6652}},
volume = {{85}},
year = {{1988}},
}