Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis
(2018) In BMC Pulmonary Medicine 18(1).- Abstract
Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease... (More)
Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease in FEV1%predicted compared to the patients' individual baseline pulmonary function (ΔFEV1), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of > 10% decrease in ΔFEV1 were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load. Conclusions: Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.
(Less)
- author
- Hovold, Gisela LU ; Palmcrantz, Victoria ; Kahn, Fredrik LU ; Egesten, Arne LU and Påhlman, Lisa I. LU
- organization
- publishing date
- 2018-06-20
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Airway infection, Children, Cystic fibrosis, Inflammation, Lung function, Sputum
- in
- BMC Pulmonary Medicine
- volume
- 18
- issue
- 1
- article number
- 104
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:85048869257
- pmid:29925362
- ISSN
- 1471-2466
- DOI
- 10.1186/s12890-018-0668-7
- language
- English
- LU publication?
- yes
- id
- 1a358d9d-9114-49cf-8bc6-6d3877a962dc
- date added to LUP
- 2018-07-05 12:44:00
- date last changed
- 2024-04-01 06:08:59
@article{1a358d9d-9114-49cf-8bc6-6d3877a962dc, abstract = {{<p>Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease in FEV<sub>1</sub>%predicted compared to the patients' individual baseline pulmonary function (ΔFEV<sub>1</sub>), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of > 10% decrease in ΔFEV<sub>1</sub> were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load. Conclusions: Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.</p>}}, author = {{Hovold, Gisela and Palmcrantz, Victoria and Kahn, Fredrik and Egesten, Arne and Påhlman, Lisa I.}}, issn = {{1471-2466}}, keywords = {{Airway infection; Children; Cystic fibrosis; Inflammation; Lung function; Sputum}}, language = {{eng}}, month = {{06}}, number = {{1}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Pulmonary Medicine}}, title = {{Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis}}, url = {{http://dx.doi.org/10.1186/s12890-018-0668-7}}, doi = {{10.1186/s12890-018-0668-7}}, volume = {{18}}, year = {{2018}}, }