A Role for Wnt Signaling in the Reversal of Metabolic Disturbances after Gastric Bypass in Obese Mice
(2015) 75th Scientific Sessions of the American Diabetes Association In Diabetes 64(Suppl 1). p.546-546- Abstract
- Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt
genes are both positively and negatively correlated with obesity-induced
adipose tissue infl ammation and insulin resistance, our main aim is to reveal
a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.
8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for
14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham
surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet
for another 8 weeks (Sham group was pair fed with the RYGB group). Using
... (More) - Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt
genes are both positively and negatively correlated with obesity-induced
adipose tissue infl ammation and insulin resistance, our main aim is to reveal
a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.
8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for
14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham
surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet
for another 8 weeks (Sham group was pair fed with the RYGB group). Using
qRT-PCR, the mRNA expression profi le of Wnt ligands and its antagonists
(i.e. secreted Frizzled-related proteins (sFRP)) was determined in epididymal
white adipose tissue of RYGB and Sham mice.
8 weeks post-surgery, Sham mice returned to their pre-surgery weight
(43.4 g ± 6.9), whereas RYGB mice showed a 43% reduction in body weight,
accompanied by improved metabolic parameters, as compared to Sham mice
(P < 0.01). Epididymal white adipose tissue of RYGB mice showed a 75% and
94% decrease in sFRP4 and sFRP5 mRNA expression levels respectively as
compared to Sham mice (P <0.01). Additionally, a trend towards decreased
mRNA expression of Wnt5a and Wnt5b could be detected in RYGB mice as
compared to Sham mice (P = 0.1).
In conclusion, RYGB surgery strongly reduced the expression level of the
secreted Wnt antagonists sFRP4 and sFRP5 in white adipocyte tissue. As
secreted proteins are interesting drug targets, a potential role for these secreted proteins in the reversal of metabolic disturbances after RYGB surgery
will be studied more in depth.
Supported By: FWOG.0857.13 (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1c730618-3d80-423b-b153-d8c3940b8c36
- author
- Vangoitsenhoven, Roman ; Van der Schueren, Bart ; da Cunha, Joao Paulo LU ; Lannoo, Matthias ; Mathieu, Chantal ; Overbergh, Lut and Maris, Michael
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- in
- Diabetes
- volume
- 64
- issue
- Suppl 1
- pages
- 546 - 546
- publisher
- American Diabetes Association Inc.
- conference name
- 75th Scientific Sessions of the American Diabetes Association
- conference location
- Boston, United States
- conference dates
- 2015-06-05 - 2015-06-09
- ISSN
- 1939-327X
- DOI
- 10.2337/db15-1929-2253
- language
- English
- LU publication?
- no
- id
- 1c730618-3d80-423b-b153-d8c3940b8c36
- date added to LUP
- 2020-01-30 16:36:42
- date last changed
- 2020-02-04 13:17:37
@misc{1c730618-3d80-423b-b153-d8c3940b8c36, abstract = {{Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt<br> genes are both positively and negatively correlated with obesity-induced<br> adipose tissue infl ammation and insulin resistance, our main aim is to reveal<br> a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.<br> 8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for<br> 14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham<br> surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet<br> for another 8 weeks (Sham group was pair fed with the RYGB group). Using<br> qRT-PCR, the mRNA expression profi le of Wnt ligands and its antagonists<br> (i.e. secreted Frizzled-related proteins (sFRP)) was determined in epididymal<br> white adipose tissue of RYGB and Sham mice.<br> 8 weeks post-surgery, Sham mice returned to their pre-surgery weight<br> (43.4 g ± 6.9), whereas RYGB mice showed a 43% reduction in body weight,<br> accompanied by improved metabolic parameters, as compared to Sham mice<br> (P < 0.01). Epididymal white adipose tissue of RYGB mice showed a 75% and<br> 94% decrease in sFRP4 and sFRP5 mRNA expression levels respectively as<br> compared to Sham mice (P <0.01). Additionally, a trend towards decreased<br> mRNA expression of Wnt5a and Wnt5b could be detected in RYGB mice as<br> compared to Sham mice (P = 0.1).<br> In conclusion, RYGB surgery strongly reduced the expression level of the<br> secreted Wnt antagonists sFRP4 and sFRP5 in white adipocyte tissue. As<br> secreted proteins are interesting drug targets, a potential role for these secreted proteins in the reversal of metabolic disturbances after RYGB surgery<br> will be studied more in depth.<br> Supported By: FWOG.0857.13}}, author = {{Vangoitsenhoven, Roman and Van der Schueren, Bart and da Cunha, Joao Paulo and Lannoo, Matthias and Mathieu, Chantal and Overbergh, Lut and Maris, Michael}}, issn = {{1939-327X}}, language = {{eng}}, note = {{Conference Abstract}}, number = {{Suppl 1}}, pages = {{546--546}}, publisher = {{American Diabetes Association Inc.}}, series = {{Diabetes}}, title = {{A Role for Wnt Signaling in the Reversal of Metabolic Disturbances after Gastric Bypass in Obese Mice}}, url = {{http://dx.doi.org/10.2337/db15-1929-2253}}, doi = {{10.2337/db15-1929-2253}}, volume = {{64}}, year = {{2015}}, }