Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

High-performance liquid affinity chromatography of proteins on Cibacron Blue F3G-A bonded silica

Lowe, Christopher R. ; Glad, Magnus LU ; Larsson, Per-Olof LU ; Ohlson, Sten ; Small, David A. P. ; Atkinson, Tony and Mosbach, Klaus LU (1981) In Journal of chromatography. A 215. p.303-316
Abstract
The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.

Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic... (More)
The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.

Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic ribonuclease A from simple or complex mixtures. The examples demonstrate the versatility of HPLAC on silica-immobilised Cibacron Blue F3G-A. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cibacron Blue F3G-A, 6-aminohexyl derivative, porous silica
in
Journal of chromatography. A
volume
215
pages
14 pages
publisher
Elsevier
external identifiers
  • scopus:0019804741
ISSN
0021-9673
DOI
10.1016/S0021-9673(00)81409-0
language
English
LU publication?
yes
id
1ef6f842-489a-449d-b254-d652a9d36fab
date added to LUP
2024-06-18 20:35:02
date last changed
2024-09-06 15:46:02
@article{1ef6f842-489a-449d-b254-d652a9d36fab,
  abstract     = {{The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.<br/><br/>Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic ribonuclease A from simple or complex mixtures. The examples demonstrate the versatility of HPLAC on silica-immobilised Cibacron Blue F3G-A.}},
  author       = {{Lowe, Christopher R. and Glad, Magnus and Larsson, Per-Olof and Ohlson, Sten and Small, David A. P. and Atkinson, Tony and Mosbach, Klaus}},
  issn         = {{0021-9673}},
  keywords     = {{Cibacron Blue F3G-A; 6-aminohexyl derivative; porous silica}},
  language     = {{eng}},
  pages        = {{303--316}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{High-performance liquid affinity chromatography of proteins on Cibacron Blue F3G-A bonded silica}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(00)81409-0}},
  doi          = {{10.1016/S0021-9673(00)81409-0}},
  volume       = {{215}},
  year         = {{1981}},
}