High-performance liquid affinity chromatography of proteins on Cibacron Blue F3G-A bonded silica
(1981) In Journal of chromatography. A 215. p.303-316- Abstract
- The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.
Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic... (More) - The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.
Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic ribonuclease A from simple or complex mixtures. The examples demonstrate the versatility of HPLAC on silica-immobilised Cibacron Blue F3G-A. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1ef6f842-489a-449d-b254-d652a9d36fab
- author
- Lowe, Christopher R. ; Glad, Magnus LU ; Larsson, Per-Olof LU ; Ohlson, Sten ; Small, David A. P. ; Atkinson, Tony and Mosbach, Klaus LU
- organization
- publishing date
- 1981
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cibacron Blue F3G-A, 6-aminohexyl derivative, porous silica
- in
- Journal of chromatography. A
- volume
- 215
- pages
- 14 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0019804741
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(00)81409-0
- language
- English
- LU publication?
- yes
- id
- 1ef6f842-489a-449d-b254-d652a9d36fab
- date added to LUP
- 2024-06-18 20:35:02
- date last changed
- 2024-09-06 15:46:02
@article{1ef6f842-489a-449d-b254-d652a9d36fab, abstract = {{The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated porous silica (5 μm) to generate an adsorbent containing 5.5–6.7 μmol dye/g silica.<br/><br/>Cibacron Blue F3G-A silica columns, in conjunction with on-line monitoring of protein concentration and enzyme activity, may be exploited for the high speed fully automated resolution of dehydrogenases, isoenzymes, kinases and other proteins such as pancreatic ribonuclease A from simple or complex mixtures. The examples demonstrate the versatility of HPLAC on silica-immobilised Cibacron Blue F3G-A.}}, author = {{Lowe, Christopher R. and Glad, Magnus and Larsson, Per-Olof and Ohlson, Sten and Small, David A. P. and Atkinson, Tony and Mosbach, Klaus}}, issn = {{0021-9673}}, keywords = {{Cibacron Blue F3G-A; 6-aminohexyl derivative; porous silica}}, language = {{eng}}, pages = {{303--316}}, publisher = {{Elsevier}}, series = {{Journal of chromatography. A}}, title = {{High-performance liquid affinity chromatography of proteins on Cibacron Blue F3G-A bonded silica}}, url = {{http://dx.doi.org/10.1016/S0021-9673(00)81409-0}}, doi = {{10.1016/S0021-9673(00)81409-0}}, volume = {{215}}, year = {{1981}}, }