High-capacity composite adsorbents for nucleic acids.
(2011) In Journal of chromatography. A 1218(31). p.5235-5240- Abstract
- Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore... (More)
- Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore flow was in operation. The dynamic binding capacities (10% breakthrough) per gram of dry weight Cytopore were determined for several compounds including DNA and RNA and were found to be 240-370mg/g. The composite was used to isolate pUC 18-type plasmids from a cleared alkaline lysate in a good yield. Confocal microscopy studies showed that plasmids were bound not only to the surface of the Cytopore material but also within the matrix walls, thus offering an explanation to the very high binding capacities observed. The concept of using a composite prepared from a mechanically weak, high-binding material and a strong scaffold material may be applied to other systems as well. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2059210
- author
- Tiainen, Peter LU ; Rokebul Anower, M and Larsson, Per-Olof LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of chromatography. A
- volume
- 1218
- issue
- 31
- pages
- 5235 - 5240
- publisher
- Elsevier
- external identifiers
-
- wos:000293432200025
- pmid:21719019
- scopus:79960112275
- pmid:21719019
- ISSN
- 1873-3778
- DOI
- 10.1016/j.chroma.2011.06.001
- language
- English
- LU publication?
- yes
- id
- b1b59faf-3dfe-44ac-a0a7-18fcb0d106cb (old id 2059210)
- date added to LUP
- 2016-04-01 12:55:56
- date last changed
- 2022-02-04 01:23:49
@article{b1b59faf-3dfe-44ac-a0a7-18fcb0d106cb,
abstract = {{Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore flow was in operation. The dynamic binding capacities (10% breakthrough) per gram of dry weight Cytopore were determined for several compounds including DNA and RNA and were found to be 240-370mg/g. The composite was used to isolate pUC 18-type plasmids from a cleared alkaline lysate in a good yield. Confocal microscopy studies showed that plasmids were bound not only to the surface of the Cytopore material but also within the matrix walls, thus offering an explanation to the very high binding capacities observed. The concept of using a composite prepared from a mechanically weak, high-binding material and a strong scaffold material may be applied to other systems as well.}},
author = {{Tiainen, Peter and Rokebul Anower, M and Larsson, Per-Olof}},
issn = {{1873-3778}},
language = {{eng}},
number = {{31}},
pages = {{5235--5240}},
publisher = {{Elsevier}},
series = {{Journal of chromatography. A}},
title = {{High-capacity composite adsorbents for nucleic acids.}},
url = {{http://dx.doi.org/10.1016/j.chroma.2011.06.001}},
doi = {{10.1016/j.chroma.2011.06.001}},
volume = {{1218}},
year = {{2011}},
}