Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets
(2005) In AIDS Research and Human Retroviruses 21(12). p.1003-1010- Abstract
- Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as... (More)
- Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets. (Less)
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https://lup.lub.lu.se/record/210046
- author
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- AIDS Research and Human Retroviruses
- volume
- 21
- issue
- 12
- pages
- 1003 - 1010
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- pmid:16379603
- wos:000234570100004
- scopus:30344455310
- ISSN
- 1931-8405
- DOI
- 10.1089/aid.2005.21.1003
- language
- English
- LU publication?
- yes
- id
- 1bfaf06c-78c4-46df-8a0d-48c872f2ddb8 (old id 210046)
- date added to LUP
- 2016-04-01 12:13:48
- date last changed
- 2022-01-27 00:44:09
@article{1bfaf06c-78c4-46df-8a0d-48c872f2ddb8, abstract = {{Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets.}}, author = {{Casper, C and Mild, Mattias and Jansson, Marianne and Karlsson, A and Holmberg, V and Bratt, G and van Paaschen, H and Biberfeld, P and Bjorndal, A and Albert, J and Popovic, M and Fenyö, Eva Maria}}, issn = {{1931-8405}}, language = {{eng}}, number = {{12}}, pages = {{1003--1010}}, publisher = {{Mary Ann Liebert, Inc.}}, series = {{AIDS Research and Human Retroviruses}}, title = {{Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets}}, url = {{http://dx.doi.org/10.1089/aid.2005.21.1003}}, doi = {{10.1089/aid.2005.21.1003}}, volume = {{21}}, year = {{2005}}, }