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Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics

Akerblad, P ; Månsson, Robert LU ; Lagergren, Anna LU ; Westerlund, S ; Basta, B ; Lind, U ; Thelin, A ; Gisler, Ramiro LU ; Liberg, David LU and Nelander, S , et al. (2005) In Physiological Genomics 23(2). p.206-216
Abstract
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR... (More)
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
early B cell factor, peroxisome proliferator-activated, adipogenesis, receptor-gamma 2
in
Physiological Genomics
volume
23
issue
2
pages
206 - 216
publisher
American Physiological Society
external identifiers
  • wos:000234086200009
  • scopus:33646898028
ISSN
1094-8341
DOI
10.1152/physiolgenomics.00015.2005
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012)
id
725e1f56-b45f-431d-ab4d-856628d6af72 (old id 210428)
date added to LUP
2016-04-01 12:29:13
date last changed
2025-10-14 11:34:41
@article{725e1f56-b45f-431d-ab4d-856628d6af72,
  abstract     = {{Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.}},
  author       = {{Akerblad, P and Månsson, Robert and Lagergren, Anna and Westerlund, S and Basta, B and Lind, U and Thelin, A and Gisler, Ramiro and Liberg, David and Nelander, S and Bamberg, K and Sigvardsson, Mikael}},
  issn         = {{1094-8341}},
  keywords     = {{early B cell factor; peroxisome proliferator-activated; adipogenesis; receptor-gamma 2}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{206--216}},
  publisher    = {{American Physiological Society}},
  series       = {{Physiological Genomics}},
  title        = {{Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics}},
  url          = {{http://dx.doi.org/10.1152/physiolgenomics.00015.2005}},
  doi          = {{10.1152/physiolgenomics.00015.2005}},
  volume       = {{23}},
  year         = {{2005}},
}