Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring
(2005) In Biosensors & Bioelectronics 21(5). p.742-748- Abstract
- Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is... (More)
- Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/214038
- author
- Alpeeva, IS ; Nistor, Mihaela LU ; Castillo Leon, Jaime LU ; Csöregi, Elisabeth LU and Sakharova, IY
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- sweet potato, horseradish, palm tree, peroxidase, biosensors, hydrogen, peroxide
- in
- Biosensors & Bioelectronics
- volume
- 21
- issue
- 5
- pages
- 742 - 748
- publisher
- Elsevier
- external identifiers
-
- wos:000233154300008
- pmid:16242613
- scopus:26944481128
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2005.01.008
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 38f55db1-9f6b-4636-af5f-7e8ca61d84b6 (old id 214038)
- date added to LUP
- 2016-04-01 16:48:45
- date last changed
- 2022-01-28 22:17:48
@article{38f55db1-9f6b-4636-af5f-7e8ca61d84b6, abstract = {{Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.}}, author = {{Alpeeva, IS and Nistor, Mihaela and Castillo Leon, Jaime and Csöregi, Elisabeth and Sakharova, IY}}, issn = {{1873-4235}}, keywords = {{sweet potato; horseradish; palm tree; peroxidase; biosensors; hydrogen; peroxide}}, language = {{eng}}, number = {{5}}, pages = {{742--748}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring}}, url = {{http://dx.doi.org/10.1016/j.bios.2005.01.008}}, doi = {{10.1016/j.bios.2005.01.008}}, volume = {{21}}, year = {{2005}}, }