Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs.
Hult, Annika LU ; Frame, Tom ; Chesla, Scott ; Henry, Stephen and Olsson, Martin L LU
(2012)
In Transfusion
52.
p.247-251
- Abstract
- BACKGROUND:
Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.
STUDY DESIGN AND METHODS:
A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of... (More) - BACKGROUND:
Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.
STUDY DESIGN AND METHODS:
A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells.
RESULTS:
Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL-A or 78 to 10 µg/mL FSL-B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native A(x) and most B(weak) subgroups.
CONCLUSIONS:
Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2151561
- author
- Hult, Annika
LU
; Frame, Tom
; Chesla, Scott
; Henry, Stephen
and Olsson, Martin L
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion
- volume
- 52
- pages
- 247 - 251
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000299038200009
- pmid:21812783
- scopus:84855851546
- pmid:21812783
- ISSN
- 1537-2995
- DOI
- 10.1111/j.1537-2995.2011.03268.x
- language
- English
- LU publication?
- yes
- id
- 2d60fa8b-9f93-454e-b224-231e175b8e10 (old id 2151561)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21812783?dopt=Abstract
- date added to LUP
- 2016-04-04 09:33:48
- date last changed
- 2022-02-28 08:35:14
@article{2d60fa8b-9f93-454e-b224-231e175b8e10,
abstract = {{BACKGROUND:<br/><br>
Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.<br/><br>
<br/><br>
STUDY DESIGN AND METHODS:<br/><br>
A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells.<br/><br>
<br/><br>
RESULTS:<br/><br>
Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL-A or 78 to 10 µg/mL FSL-B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native A(x) and most B(weak) subgroups.<br/><br>
<br/><br>
CONCLUSIONS:<br/><br>
Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs.}},
author = {{Hult, Annika and Frame, Tom and Chesla, Scott and Henry, Stephen and Olsson, Martin L}},
issn = {{1537-2995}},
language = {{eng}},
pages = {{247--251}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion}},
title = {{Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs.}},
url = {{http://dx.doi.org/10.1111/j.1537-2995.2011.03268.x}},
doi = {{10.1111/j.1537-2995.2011.03268.x}},
volume = {{52}},
year = {{2012}},
}