Imaging apolipoprotein AI in vivo
(2011) In NMR in Biomedicine 24(7). p.916-924- Abstract
- Coronary disease risk increases inversely with high-density lipoprotein (HDL) level. The measurement of the biodistribution and clearance of HDL in vivo, however, has posed a technical challenge. This study presents an approach to the development of a lipoprotein MRI agent by linking gadolinium methanethiosulfonate (Gd[MTS-ADO3A]) to a selective cysteine mutation in position 55 of apo AI, the major protein of HDL. The contrast agent targets both liver and kidney, the sites of HDL catabolism, whereas the standard MRI contrast agent, gadolinium-diethylenetriaminepentaacetic acid-bismethylamide (GdDTPA-BMA, gadodiamide), enhances only the kidney image. Using a modified apolipoprotein AI to create an HDL contrast agent provides a new approach... (More)
- Coronary disease risk increases inversely with high-density lipoprotein (HDL) level. The measurement of the biodistribution and clearance of HDL in vivo, however, has posed a technical challenge. This study presents an approach to the development of a lipoprotein MRI agent by linking gadolinium methanethiosulfonate (Gd[MTS-ADO3A]) to a selective cysteine mutation in position 55 of apo AI, the major protein of HDL. The contrast agent targets both liver and kidney, the sites of HDL catabolism, whereas the standard MRI contrast agent, gadolinium-diethylenetriaminepentaacetic acid-bismethylamide (GdDTPA-BMA, gadodiamide), enhances only the kidney image. Using a modified apolipoprotein AI to create an HDL contrast agent provides a new approach to investigate HDL biodistribution, metabolism and regulation in vivo. Copyright (C) 2011 John Wiley & Sons, Ltd. (Less)
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https://lup.lub.lu.se/record/2186757
- author
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NMR, apolipoprotein, HDL, contrast agent, MRI, gadolinium, cardiovascular risk
- in
- NMR in Biomedicine
- volume
- 24
- issue
- 7
- pages
- 916 - 924
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000294686900019
- scopus:80051581209
- pmid:21264979
- ISSN
- 0952-3480
- DOI
- 10.1002/nbm.1650
- language
- English
- LU publication?
- yes
- id
- 2adc16c8-5aeb-4cd2-8c62-06ecdffd285b (old id 2186757)
- date added to LUP
- 2016-04-01 10:15:26
- date last changed
- 2022-03-19 19:02:46
@article{2adc16c8-5aeb-4cd2-8c62-06ecdffd285b, abstract = {{Coronary disease risk increases inversely with high-density lipoprotein (HDL) level. The measurement of the biodistribution and clearance of HDL in vivo, however, has posed a technical challenge. This study presents an approach to the development of a lipoprotein MRI agent by linking gadolinium methanethiosulfonate (Gd[MTS-ADO3A]) to a selective cysteine mutation in position 55 of apo AI, the major protein of HDL. The contrast agent targets both liver and kidney, the sites of HDL catabolism, whereas the standard MRI contrast agent, gadolinium-diethylenetriaminepentaacetic acid-bismethylamide (GdDTPA-BMA, gadodiamide), enhances only the kidney image. Using a modified apolipoprotein AI to create an HDL contrast agent provides a new approach to investigate HDL biodistribution, metabolism and regulation in vivo. Copyright (C) 2011 John Wiley & Sons, Ltd.}}, author = {{Sriram, Renuka and Lagerstedt, Jens and Petrlova, Jitka and Samardzic, Haris and Kreutzer, Ulrike and Xie, Hongtao and Kaysen, George A. and Desreux, Jean F. and Thonon, David and Jacques, Vincent and Van Loan, Martha and Rutledge, John C. and Oda, Michael N. and Voss, John C. and Jue, Thomas}}, issn = {{0952-3480}}, keywords = {{NMR; apolipoprotein; HDL; contrast agent; MRI; gadolinium; cardiovascular risk}}, language = {{eng}}, number = {{7}}, pages = {{916--924}}, publisher = {{John Wiley & Sons Inc.}}, series = {{NMR in Biomedicine}}, title = {{Imaging apolipoprotein AI in vivo}}, url = {{http://dx.doi.org/10.1002/nbm.1650}}, doi = {{10.1002/nbm.1650}}, volume = {{24}}, year = {{2011}}, }