PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration
(1984) In Nuclear Instruments and Methods in Physics Research B 3(1-3). p.373-376- Abstract
- Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.
From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.
By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron... (More) - Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.
From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.
By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2204943
- author
- Pallon, Jan LU ; Pakarinen, Pirjo and Akselsson, Roland LU
- organization
- publishing date
- 1984
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- serum proteins, gel filtration, PIXE, elemental composition
- in
- Nuclear Instruments and Methods in Physics Research B
- volume
- 3
- issue
- 1-3
- pages
- 373 - 376
- publisher
- North-Holland
- external identifiers
-
- scopus:25744477027
- DOI
- 10.1016/0168-583X(84)90398-7
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Ergonomics and Aerosol Technology (011025002)
- id
- b9508519-8e87-4bac-8dcc-638247c5a07d (old id 2204943)
- date added to LUP
- 2016-04-04 12:04:28
- date last changed
- 2021-01-03 07:43:48
@article{b9508519-8e87-4bac-8dcc-638247c5a07d, abstract = {{Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.<br/><br> From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.<br/><br> By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements.}}, author = {{Pallon, Jan and Pakarinen, Pirjo and Akselsson, Roland}}, keywords = {{serum proteins; gel filtration; PIXE; elemental composition}}, language = {{eng}}, number = {{1-3}}, pages = {{373--376}}, publisher = {{North-Holland}}, series = {{Nuclear Instruments and Methods in Physics Research B}}, title = {{PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration}}, url = {{http://dx.doi.org/10.1016/0168-583X(84)90398-7}}, doi = {{10.1016/0168-583X(84)90398-7}}, volume = {{3}}, year = {{1984}}, }