Sampling, sample treatment, assay and real time monitoring of biotechnological processes using an integrated flow-injection system
(2011)- Abstract
- Microorganisms can be used for production of pharmaceutical substances. With the
introduction of gene technology, it is possible to transfer information from one
organism to the other, thereby making the receiving organism capable of producing
substances that it did not so before. This development is very important for e.g.
production of therapeutic proteins
However, when using living organisms for production there is a need for controlling
a range of different parameters. In order to understand the process it is necessary
to have as much information as possible and then an algorithm to analyze the data.
In this thesis work is presented that addresses some of the... (More) - Microorganisms can be used for production of pharmaceutical substances. With the
introduction of gene technology, it is possible to transfer information from one
organism to the other, thereby making the receiving organism capable of producing
substances that it did not so before. This development is very important for e.g.
production of therapeutic proteins
However, when using living organisms for production there is a need for controlling
a range of different parameters. In order to understand the process it is necessary
to have as much information as possible and then an algorithm to analyze the data.
In this thesis work is presented that addresses some of the bottlenecks in
development of process monitoring. The coaxial catheter was evaluated with regard
to sampling from complex, sensitive medium without leakage of the inhibitory
solution that is used for instantaneous stopping of ongoing enzymatic/microbial
processes in the sample.
An automated system for advanced flow injection analysis of complex
biotechnological processes has been constructed and it was used to evaluate
efficiency of different inducer combinations when expressing a clone of enzyme.
One key factor in flow injection analysis is to prevent flow problems. The
introduction of supermacroporous hydrogels made it possible to introduce whole
broth from a fermenter into the system and also to include a superporous
catalytically active gel composed of microbial cells directly cross-linked to each
other.
Even if there is a need, still very few successful examples are presented where
advanced monitoring is used to control the processes. The missing part here is the
lack of a reliable, representative and robust sampling and sample handling system,
which is compatible with analytical devices.
The objective of this thesis work was to develop an aseptic and representative
sampling unit and treatment of the bioreactor product in the flow system, and
finally, analysing the sample by enzymatic and immunoassays in the integrated flow
system. (Less) - Abstract (Swedish)
- Popular Abstract in English
Microorganisms can be used for production of pharmaceutical substances. With the
introduction of gene technology, it is possible to transfer information from one
organism to the other, thereby making the receiving organism capable of producing
substances that it did not so before. This development is very important for e.g.
production of therapeutic proteins
However, when using living organisms for production there is a need for controlling
a range of different parameters. In order to understand the process it is necessary
to have as much information as possible and then an algorithm to analyze the data.
In this thesis work... (More) - Popular Abstract in English
Microorganisms can be used for production of pharmaceutical substances. With the
introduction of gene technology, it is possible to transfer information from one
organism to the other, thereby making the receiving organism capable of producing
substances that it did not so before. This development is very important for e.g.
production of therapeutic proteins
However, when using living organisms for production there is a need for controlling
a range of different parameters. In order to understand the process it is necessary
to have as much information as possible and then an algorithm to analyze the data.
In this thesis work is presented that addresses some of the bottlenecks in
development of process monitoring. The coaxial catheter was evaluated with regard
to sampling from complex, sensitive medium without leakage of the inhibitory
solution that is used for instantaneous stopping of ongoing enzymatic/microbial
processes in the sample.
An automated system for advanced flow injection analysis of complex
biotechnological processes has been constructed and it was used to evaluate
efficiency of different inducer combinations when expressing a clone of enzyme.
One key factor in flow injection analysis is to prevent flow problems. The
introduction of supermacroporous hydrogels made it possible to introduce whole
broth from a fermenter into the system and also to include a superporous
catalytically active gel composed of microbial cells directly cross-linked to each
other.
Even if there is a need, still very few successful examples are presented where
advanced monitoring is used to control the processes. The missing part here is the
lack of a reliable, representative and robust sampling and sample handling system,
which is compatible with analytical devices.
The objective of this thesis work was to develop an aseptic and representative
sampling unit and treatment of the bioreactor product in the flow system, and
finally, analysing the sample by enzymatic and immunoassays in the integrated flow
system. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2205316
- author
- Mazlomi, Mohammad LU
- supervisor
-
- Bo Mattiasson LU
- Martin Hedström LU
- opponent
-
- Professor Håkansson, Håkan, GS Development, Malmö
- organization
- publishing date
- 2011
- type
- Thesis
- publication status
- published
- subject
- keywords
- Bioprocess, Sampling, Sample treatment, On-line monitoring, Real time monitoring, Flow injection, Immunoassay, Fermentation
- pages
- 132 pages
- defense location
- Lecture Hall B, Kemicentrum, Getingevägen 60, Lund University, Faculty of Engineering
- defense date
- 2011-12-15 10:30:00
- ISBN
- 978-91-89627-79-6
- language
- English
- LU publication?
- yes
- id
- 516296ad-c0dd-46b2-9ff5-5c593a71e343 (old id 2205316)
- date added to LUP
- 2016-04-04 14:38:18
- date last changed
- 2018-11-21 21:21:27
@phdthesis{516296ad-c0dd-46b2-9ff5-5c593a71e343, abstract = {{Microorganisms can be used for production of pharmaceutical substances. With the<br/><br> introduction of gene technology, it is possible to transfer information from one<br/><br> organism to the other, thereby making the receiving organism capable of producing<br/><br> substances that it did not so before. This development is very important for e.g.<br/><br> production of therapeutic proteins<br/><br> However, when using living organisms for production there is a need for controlling<br/><br> a range of different parameters. In order to understand the process it is necessary<br/><br> to have as much information as possible and then an algorithm to analyze the data.<br/><br> In this thesis work is presented that addresses some of the bottlenecks in<br/><br> development of process monitoring. The coaxial catheter was evaluated with regard<br/><br> to sampling from complex, sensitive medium without leakage of the inhibitory<br/><br> solution that is used for instantaneous stopping of ongoing enzymatic/microbial<br/><br> processes in the sample.<br/><br> An automated system for advanced flow injection analysis of complex<br/><br> biotechnological processes has been constructed and it was used to evaluate<br/><br> efficiency of different inducer combinations when expressing a clone of enzyme.<br/><br> One key factor in flow injection analysis is to prevent flow problems. The<br/><br> introduction of supermacroporous hydrogels made it possible to introduce whole<br/><br> broth from a fermenter into the system and also to include a superporous<br/><br> catalytically active gel composed of microbial cells directly cross-linked to each<br/><br> other.<br/><br> Even if there is a need, still very few successful examples are presented where<br/><br> advanced monitoring is used to control the processes. The missing part here is the<br/><br> lack of a reliable, representative and robust sampling and sample handling system,<br/><br> which is compatible with analytical devices.<br/><br> The objective of this thesis work was to develop an aseptic and representative<br/><br> sampling unit and treatment of the bioreactor product in the flow system, and<br/><br> finally, analysing the sample by enzymatic and immunoassays in the integrated flow<br/><br> system.}}, author = {{Mazlomi, Mohammad}}, isbn = {{978-91-89627-79-6}}, keywords = {{Bioprocess; Sampling; Sample treatment; On-line monitoring; Real time monitoring; Flow injection; Immunoassay; Fermentation}}, language = {{eng}}, school = {{Lund University}}, title = {{Sampling, sample treatment, assay and real time monitoring of biotechnological processes using an integrated flow-injection system}}, url = {{https://lup.lub.lu.se/search/files/6406446/2205321.pdf}}, year = {{2011}}, }