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Real-time Fluorescence Imaging of Capillary Electrophoresis

Nilsson, S ; Johansson, Jonas LU ; Mecklenburg, M ; Birnbaum, S ; Svanberg, Sune LU ; Wahlund, K. G ; Mosbach, K ; Miyabayashi, A and Larsson, P. O LU (1995) In Journal of Capillary Electrophoresis 2(1). p.46-52
Abstract
An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples.... (More)
An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrophoresis, Real-time imaging
in
Journal of Capillary Electrophoresis
volume
2
issue
1
pages
7 pages
publisher
International Scientific Communications Inc.
ISSN
1079-5383
language
English
LU publication?
yes
id
b98797fc-bc4a-4878-9f45-7674afc3ef97 (old id 2259079)
date added to LUP
2016-04-04 09:21:09
date last changed
2024-06-17 14:29:48
@article{b98797fc-bc4a-4878-9f45-7674afc3ef97,
  abstract     = {{An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed.}},
  author       = {{Nilsson, S and Johansson, Jonas and Mecklenburg, M and Birnbaum, S and Svanberg, Sune and Wahlund, K. G and Mosbach, K and Miyabayashi, A and Larsson, P. O}},
  issn         = {{1079-5383}},
  keywords     = {{Capillary electrophoresis; Real-time imaging}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{46--52}},
  publisher    = {{International Scientific Communications Inc.}},
  series       = {{Journal of Capillary Electrophoresis}},
  title        = {{Real-time Fluorescence Imaging of Capillary Electrophoresis}},
  volume       = {{2}},
  year         = {{1995}},
}