A simplified approach to prepare controlled cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage
(2020) In Journal of Materials Science: Materials in Medicine 31.- Abstract
- In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am... (More)
- In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2285df29-dfc1-4169-a6fc-2581672e07d4
- author
- Porras Hernández, Ana María ; Pohlit, Hannah ; Sjögren, Frida ; Shi, L ; Ossipov, D ; Antfolk, Maria LU and Tenje, Maria LU
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Materials Science: Materials in Medicine
- volume
- 31
- article number
- 89
- publisher
- Springer
- external identifiers
-
- scopus:85092551495
- ISSN
- 1573-4838
- DOI
- 10.1007/s10856-020-06446-x
- language
- English
- LU publication?
- no
- id
- 2285df29-dfc1-4169-a6fc-2581672e07d4
- date added to LUP
- 2024-04-04 19:20:29
- date last changed
- 2024-06-06 04:02:24
@article{2285df29-dfc1-4169-a6fc-2581672e07d4, abstract = {{In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.}}, author = {{Porras Hernández, Ana María and Pohlit, Hannah and Sjögren, Frida and Shi, L and Ossipov, D and Antfolk, Maria and Tenje, Maria}}, issn = {{1573-4838}}, language = {{eng}}, publisher = {{Springer}}, series = {{Journal of Materials Science: Materials in Medicine}}, title = {{A simplified approach to prepare controlled cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage}}, url = {{http://dx.doi.org/10.1007/s10856-020-06446-x}}, doi = {{10.1007/s10856-020-06446-x}}, volume = {{31}}, year = {{2020}}, }