Cytotoxicity and apoptotic effects of microcystin-LR and anatoxin-a in mouse lymphocytes
(2005) In Folia Biologica 51(3). p.62-67- Abstract
- There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed... (More)
- There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed cytotoxic effects on both lymphocyte subpopulations, but the effects were driven by mechanisms different from apoptosis. These findings demonstrate that the cyanotoxins could cause cytotoxic alterations in a variety of cell types different from the major targets, operating via distinct mechanisms. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/232329
- author
- Teneva, I ; Mladenov, R ; Popov, N and Dzhambazov, Balik LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cytotoxicity, microcystin-LR, anatoxin-a, mouse lymphocytes, apoptosis
- in
- Folia Biologica
- volume
- 51
- issue
- 3
- pages
- 62 - 67
- publisher
- Academy of Sciences of the Czech Republic
- external identifiers
-
- wos:000230552400002
- pmid:16045237
- scopus:22144485526
- ISSN
- 0015-5500
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 5d1ada0d-3b46-4ea9-bd15-8fe087374659 (old id 232329)
- alternative location
- http://fb.cuni.cz/Data/files/folia_biologica/volume_51_2005_3/FB2005A0012.pdf
- date added to LUP
- 2016-04-01 15:30:53
- date last changed
- 2022-04-22 08:03:50
@article{5d1ada0d-3b46-4ea9-bd15-8fe087374659,
abstract = {{There is an increasing amount of knowledge on the cytotoxic properties of cyanotoxins, but relatively little is known regarding their fine specificity and mechanisms of action. In this study, we investigated the influence of microcystin-LR and AnTx-a on mouse B- and T-lymphocyte subpopulations in vitro. Cyanotoxins significantly decreased the cell viability after 4 and 24 h, compared to the untreated control. After 24 h exposure to microcystin-LR and anatoxin-a, the viability of splenocytes dropped to 23% and 57%, respectively. Our data demonstrate that microcystin-LR induced apoptosis specifically in mouse B cells, probably via the B-cell antigen receptor and mitochondrial pathway, while the T cells were not affected. AnTx-a showed cytotoxic effects on both lymphocyte subpopulations, but the effects were driven by mechanisms different from apoptosis. These findings demonstrate that the cyanotoxins could cause cytotoxic alterations in a variety of cell types different from the major targets, operating via distinct mechanisms.}},
author = {{Teneva, I and Mladenov, R and Popov, N and Dzhambazov, Balik}},
issn = {{0015-5500}},
keywords = {{cytotoxicity; microcystin-LR; anatoxin-a; mouse lymphocytes; apoptosis}},
language = {{eng}},
number = {{3}},
pages = {{62--67}},
publisher = {{Academy of Sciences of the Czech Republic}},
series = {{Folia Biologica}},
title = {{Cytotoxicity and apoptotic effects of microcystin-LR and anatoxin-a in mouse lymphocytes}},
url = {{http://fb.cuni.cz/Data/files/folia_biologica/volume_51_2005_3/FB2005A0012.pdf}},
volume = {{51}},
year = {{2005}},
}