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Lifestyle Intervention in Pregnant Women With Obesity Impacts Cord Blood DNA Methylation, Which Associates With Body Composition in the Offspring

Jönsson, Josefine LU orcid ; Renault, Kristina M. ; García-Calzón, Sonia LU ; Perfilyev, Alexander LU orcid ; Estampador, Angela C. LU ; Nørgaard, Kirsten ; Lind, Mads Vendelbo ; Vaag, Allan LU ; Hjort, Line LU and Michaelsen, Kim F. , et al. (2021) In Diabetes 70(4). p.854-866
Abstract

Maternal obesity may lead to epigenetic alterations in the offspring and might thereby contribute to disease later in life. We investigated whether a lifestyle intervention in pregnant women with obesity is associated with epigenetic variation in cord blood and body composition in the offspring. Genome-wide DNA methylation was analyzed in cord blood from 208 offspring from the Treatment of Obese Pregnant women (TOP)-study, which includes pregnant women with obesity randomized to lifestyle interventions comprised of physical activity with or without dietary advice versus control subjects (standard of care). DNA methylation was altered at 379 sites, annotated to 370 genes, in cord blood from offspring of mothers following a lifestyle... (More)

Maternal obesity may lead to epigenetic alterations in the offspring and might thereby contribute to disease later in life. We investigated whether a lifestyle intervention in pregnant women with obesity is associated with epigenetic variation in cord blood and body composition in the offspring. Genome-wide DNA methylation was analyzed in cord blood from 208 offspring from the Treatment of Obese Pregnant women (TOP)-study, which includes pregnant women with obesity randomized to lifestyle interventions comprised of physical activity with or without dietary advice versus control subjects (standard of care). DNA methylation was altered at 379 sites, annotated to 370 genes, in cord blood from offspring of mothers following a lifestyle intervention versus control subjects (false discovery rate [FDR] <5%) when using the Houseman reference-free method to correct for cell composition, and three of these sites were significant based on Bonferroni correction. These 370 genes are overrepresented in gene ontology terms, including response to fatty acids and adipose tissue development. Offspring of mothers included in a lifestyle intervention were born with more lean mass compared with control subjects. Methylation at 17 sites, annotated to, for example, DISC1, GBX2, HERC2, and HUWE1, partially mediates the effect of the lifestyle intervention on lean mass in the offspring (FDR <5%). Moreover, 22 methylation sites were associated with offspring BMI z scores during the first 3 years of life (P < 0.05). Overall, lifestyle interventions in pregnant women with obesity are associated with epigenetic changes in offspring, potentially influencing the offspring's lean mass and early growth.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes
volume
70
issue
4
pages
13 pages
publisher
American Diabetes Association Inc.
external identifiers
  • pmid:33431374
  • scopus:85103306192
ISSN
1939-327X
DOI
10.2337/db20-0487
project
Do dietary factors impact the epigenome and thereby metabolism in humans?
language
English
LU publication?
yes
id
235b93f0-7f9a-4da7-9bca-cebe7fd0bbeb
date added to LUP
2021-04-07 08:38:32
date last changed
2024-04-20 04:14:08
@article{235b93f0-7f9a-4da7-9bca-cebe7fd0bbeb,
  abstract     = {{<p>Maternal obesity may lead to epigenetic alterations in the offspring and might thereby contribute to disease later in life. We investigated whether a lifestyle intervention in pregnant women with obesity is associated with epigenetic variation in cord blood and body composition in the offspring. Genome-wide DNA methylation was analyzed in cord blood from 208 offspring from the Treatment of Obese Pregnant women (TOP)-study, which includes pregnant women with obesity randomized to lifestyle interventions comprised of physical activity with or without dietary advice versus control subjects (standard of care). DNA methylation was altered at 379 sites, annotated to 370 genes, in cord blood from offspring of mothers following a lifestyle intervention versus control subjects (false discovery rate [FDR] &lt;5%) when using the Houseman reference-free method to correct for cell composition, and three of these sites were significant based on Bonferroni correction. These 370 genes are overrepresented in gene ontology terms, including response to fatty acids and adipose tissue development. Offspring of mothers included in a lifestyle intervention were born with more lean mass compared with control subjects. Methylation at 17 sites, annotated to, for example, DISC1, GBX2, HERC2, and HUWE1, partially mediates the effect of the lifestyle intervention on lean mass in the offspring (FDR &lt;5%). Moreover, 22 methylation sites were associated with offspring BMI z scores during the first 3 years of life (P &lt; 0.05). Overall, lifestyle interventions in pregnant women with obesity are associated with epigenetic changes in offspring, potentially influencing the offspring's lean mass and early growth.</p>}},
  author       = {{Jönsson, Josefine and Renault, Kristina M. and García-Calzón, Sonia and Perfilyev, Alexander and Estampador, Angela C. and Nørgaard, Kirsten and Lind, Mads Vendelbo and Vaag, Allan and Hjort, Line and Michaelsen, Kim F. and Carlsen, Emma Malchau and Franks, Paul W. and Ling, Charlotte}},
  issn         = {{1939-327X}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{854--866}},
  publisher    = {{American Diabetes Association Inc.}},
  series       = {{Diabetes}},
  title        = {{Lifestyle Intervention in Pregnant Women With Obesity Impacts Cord Blood DNA Methylation, Which Associates With Body Composition in the Offspring}},
  url          = {{http://dx.doi.org/10.2337/db20-0487}},
  doi          = {{10.2337/db20-0487}},
  volume       = {{70}},
  year         = {{2021}},
}