New information about the toxicological profile of Prorocentrum panamense (Prorocentrales, Dinophyceae) and its global distribution
(2022) In Phycological Research 70(2). p.81-88- Abstract
Dinoflagellates of the genera Prorocentrum and Dinophysis are known producers of toxic compounds belonging to the okadaic acid (OA) group. The ingestion of shellfish contaminated with these toxins causes a human disease named diarrheic shellfish poisoning (DSP). In this study, the first record of Prorocentrum panamense, a potential toxin-producer species, was reported in the Canary Islands together with its toxicological characterization. Prorocentrum panamense cells were collected during April 2017 from natural pools located in the Northeastern part of Gran Canaria. This new record represents an expansion of P. panamense distribution area, previously restricted to the Pacific Ocean, Indian Ocean, Arabian Gulf and the Caribbean, and its... (More)
Dinoflagellates of the genera Prorocentrum and Dinophysis are known producers of toxic compounds belonging to the okadaic acid (OA) group. The ingestion of shellfish contaminated with these toxins causes a human disease named diarrheic shellfish poisoning (DSP). In this study, the first record of Prorocentrum panamense, a potential toxin-producer species, was reported in the Canary Islands together with its toxicological characterization. Prorocentrum panamense cells were collected during April 2017 from natural pools located in the Northeastern part of Gran Canaria. This new record represents an expansion of P. panamense distribution area, previously restricted to the Pacific Ocean, Indian Ocean, Arabian Gulf and the Caribbean, and its introduction mechanisms are discussed. Laboratory cultures of P. panamense were settled and toxin production was assessed in both cell pellets and culture media at four different growth phases (latency, exponential, early stationary and late stationary) implementing LC-MS/MS and neuro-2a cell-based assay (CBA). LC-MS/MS allowed the identification of OA in the fraction corresponding to the late stationary phase, and tests performed on neuro-2a cells showed, for most of the fractions, OA-like activity observable by both cell morphology changes and cell mortality. This information is fundamental for a better understanding of the genus Prorocentrum global distribution, its ecology, and risks associated to toxic producing species.
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- author
- Gaiani, Greta ; Rey, María ; Tudó, Àngels LU ; Rambla, Maria ; Diogène, Jorge ; Campàs, Mònica and Alcaraz, Carles
- publishing date
- 2022-04
- type
- Contribution to journal
- publication status
- published
- keywords
- cell-based assay, central eastern Atlantic Ocean, diarrheic shellfish poisoning, okadaic acid, Prorocentrum panamense, toxin production
- in
- Phycological Research
- volume
- 70
- issue
- 2
- pages
- 8 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85124565553
- ISSN
- 1322-0829
- DOI
- 10.1111/pre.12479
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 Japanese Society of Phycology.
- id
- 235e3e7d-2b83-4335-a870-9141263d49ec
- date added to LUP
- 2024-07-09 13:12:35
- date last changed
- 2024-08-13 12:24:24
@article{235e3e7d-2b83-4335-a870-9141263d49ec,
abstract = {{<p>Dinoflagellates of the genera Prorocentrum and Dinophysis are known producers of toxic compounds belonging to the okadaic acid (OA) group. The ingestion of shellfish contaminated with these toxins causes a human disease named diarrheic shellfish poisoning (DSP). In this study, the first record of Prorocentrum panamense, a potential toxin-producer species, was reported in the Canary Islands together with its toxicological characterization. Prorocentrum panamense cells were collected during April 2017 from natural pools located in the Northeastern part of Gran Canaria. This new record represents an expansion of P. panamense distribution area, previously restricted to the Pacific Ocean, Indian Ocean, Arabian Gulf and the Caribbean, and its introduction mechanisms are discussed. Laboratory cultures of P. panamense were settled and toxin production was assessed in both cell pellets and culture media at four different growth phases (latency, exponential, early stationary and late stationary) implementing LC-MS/MS and neuro-2a cell-based assay (CBA). LC-MS/MS allowed the identification of OA in the fraction corresponding to the late stationary phase, and tests performed on neuro-2a cells showed, for most of the fractions, OA-like activity observable by both cell morphology changes and cell mortality. This information is fundamental for a better understanding of the genus Prorocentrum global distribution, its ecology, and risks associated to toxic producing species.</p>}},
author = {{Gaiani, Greta and Rey, María and Tudó, Àngels and Rambla, Maria and Diogène, Jorge and Campàs, Mònica and Alcaraz, Carles}},
issn = {{1322-0829}},
keywords = {{cell-based assay; central eastern Atlantic Ocean; diarrheic shellfish poisoning; okadaic acid; Prorocentrum panamense; toxin production}},
language = {{eng}},
number = {{2}},
pages = {{81--88}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Phycological Research}},
title = {{New information about the toxicological profile of Prorocentrum panamense (Prorocentrales, Dinophyceae) and its global distribution}},
url = {{http://dx.doi.org/10.1111/pre.12479}},
doi = {{10.1111/pre.12479}},
volume = {{70}},
year = {{2022}},
}