Laccase catalysed oxidation of syringic acid: Calorimetric determination of kinetic parameters.
(2012) In Enzyme and Microbial Technology 50(4-5). p.233-237- Abstract
- Isothermal titration calorimetry (ITC) was used to study the oxidation of syringic acid by laccases from two different sources: Galerina sp. HC1 and Trametes versicolor. Total molar heat of reaction with both enzymes was similar (230kJ/mol for Galerina laccase and 233kJ/mol for Trametes laccase), and was independent of syringic acid concentration. The kinetic parameters of the reaction were calculated from the single injection assay by applying the nonlinear least squares fitting (NLSF) of experimental data to the Michaelis-Menten equation. Higher values for V(max) were obtained with Galerina sp. laccase, whereas K(m) values were comparable for the two enzymes.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2431824
- author
- Volkova, Natalia LU ; Ibrahim, Victor LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Enzyme and Microbial Technology
- volume
- 50
- issue
- 4-5
- pages
- 233 - 237
- publisher
- Elsevier
- external identifiers
-
- wos:000303030500005
- pmid:22418263
- scopus:84858150006
- pmid:22418263
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2012.01.005
- language
- English
- LU publication?
- yes
- id
- acd6b86f-3d4f-4f73-98d5-bdb518001361 (old id 2431824)
- date added to LUP
- 2016-04-01 11:05:41
- date last changed
- 2022-04-20 17:00:31
@article{acd6b86f-3d4f-4f73-98d5-bdb518001361, abstract = {{Isothermal titration calorimetry (ITC) was used to study the oxidation of syringic acid by laccases from two different sources: Galerina sp. HC1 and Trametes versicolor. Total molar heat of reaction with both enzymes was similar (230kJ/mol for Galerina laccase and 233kJ/mol for Trametes laccase), and was independent of syringic acid concentration. The kinetic parameters of the reaction were calculated from the single injection assay by applying the nonlinear least squares fitting (NLSF) of experimental data to the Michaelis-Menten equation. Higher values for V(max) were obtained with Galerina sp. laccase, whereas K(m) values were comparable for the two enzymes.}}, author = {{Volkova, Natalia and Ibrahim, Victor and Hatti-Kaul, Rajni}}, issn = {{0141-0229}}, language = {{eng}}, number = {{4-5}}, pages = {{233--237}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{Laccase catalysed oxidation of syringic acid: Calorimetric determination of kinetic parameters.}}, url = {{http://dx.doi.org/10.1016/j.enzmictec.2012.01.005}}, doi = {{10.1016/j.enzmictec.2012.01.005}}, volume = {{50}}, year = {{2012}}, }