Immuno-affinity interactions in sample preparation and analysis
(2005)- Abstract
- Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.
In this context, a novel immuno-affinity based sample... (More) - Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.
In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/24391
- author
- Tudorache, Madalina LU
- supervisor
- opponent
-
- Professor Doctor Hennion, Marie-Claire, ESPCI (Ecole Supérieure de Physqiue et de Chimie de Paris), Laboratoire Environnement et Chimie Anal
- organization
- publishing date
- 2005
- type
- Thesis
- publication status
- published
- subject
- keywords
- immuno supported liquid membrane (ISLM) extraction, sample preparation, immunoassay, immuno-affinity interaction, antibody, Analytical chemistry, Analytisk kemi
- pages
- 156 pages
- publisher
- Analytical Chemistry, Lund University
- defense location
- Centre for Chemistry and Chemical Engineering Sölvegatan 39 Lund
- defense date
- 2005-09-02 10:30:00
- ISBN
- 91-7422-089-6
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 6d4d19a0-38be-44a5-ad11-2dc98c208b40 (old id 24391)
- date added to LUP
- 2016-04-04 10:47:48
- date last changed
- 2018-11-21 21:00:50
@phdthesis{6d4d19a0-38be-44a5-ad11-2dc98c208b40, abstract = {{Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.<br/><br> <br/><br> In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique.}}, author = {{Tudorache, Madalina}}, isbn = {{91-7422-089-6}}, keywords = {{immuno supported liquid membrane (ISLM) extraction; sample preparation; immunoassay; immuno-affinity interaction; antibody; Analytical chemistry; Analytisk kemi}}, language = {{eng}}, publisher = {{Analytical Chemistry, Lund University}}, school = {{Lund University}}, title = {{Immuno-affinity interactions in sample preparation and analysis}}, year = {{2005}}, }