Real-time label-free affinity biosensors for enumeration of total bacteria based on immobilized concanavalin A
(2011) In Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering 46(13). p.1450-1460- Abstract
- This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count... (More)
- This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count agar method. Cell concentrations obtained by the biosensor were only slightly different from the result obtained from the plate count agar. The proposed system offers a rapid and useful tool for screening detection and enumeration of total bacteria. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2494078
- author
- Jantra, Jongjit ; Kanatharana, Proespichaya ; Asawatreratanakul, Punnee ; Hedström, Martin LU ; Mattiasson, Bo LU and Thavarungkul, Panote
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacteria, surface plasmon resonance, impedance, biosensor, high, sensitivity, real-time
- in
- Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering
- volume
- 46
- issue
- 13
- pages
- 1450 - 1460
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000298320600002
- scopus:80053548556
- pmid:21961522
- ISSN
- 1093-4529
- DOI
- 10.1080/10934529.2011.609022
- language
- English
- LU publication?
- yes
- id
- d9f8fd80-ea28-4e7e-953d-aa4266822e55 (old id 2494078)
- date added to LUP
- 2016-04-01 10:55:27
- date last changed
- 2022-01-26 03:45:57
@article{d9f8fd80-ea28-4e7e-953d-aa4266822e55, abstract = {{This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on amodified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HClt at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 x 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count agar method. Cell concentrations obtained by the biosensor were only slightly different from the result obtained from the plate count agar. The proposed system offers a rapid and useful tool for screening detection and enumeration of total bacteria.}}, author = {{Jantra, Jongjit and Kanatharana, Proespichaya and Asawatreratanakul, Punnee and Hedström, Martin and Mattiasson, Bo and Thavarungkul, Panote}}, issn = {{1093-4529}}, keywords = {{Bacteria; surface plasmon resonance; impedance; biosensor; high; sensitivity; real-time}}, language = {{eng}}, number = {{13}}, pages = {{1450--1460}}, publisher = {{Taylor & Francis}}, series = {{Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering}}, title = {{Real-time label-free affinity biosensors for enumeration of total bacteria based on immobilized concanavalin A}}, url = {{http://dx.doi.org/10.1080/10934529.2011.609022}}, doi = {{10.1080/10934529.2011.609022}}, volume = {{46}}, year = {{2011}}, }