Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii
(2005) In Chinese Medical Journal 118(4). p.340-343- Abstract
- Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In... (More)
- Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In order to explore the universality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii) were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA and parC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) and sequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drug efflux were analysed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/250031
- author
- Shi, WF ; Jiang, JP ; Xu, Ning LU ; Huang, ZM and Wang, YY
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- m-chlorophenylhydrazone, carbonyl cyanide, reserpine, Acinetobacter baumanii, efflux effect, gyrA, parC
- in
- Chinese Medical Journal
- volume
- 118
- issue
- 4
- pages
- 340 - 343
- publisher
- Chinese Medical Association
- external identifiers
-
- wos:000227511600014
- pmid:15740676
- scopus:14644396616
- ISSN
- 0366-6999
- language
- English
- LU publication?
- yes
- id
- 0a6fdde9-b97b-41ae-b12c-438d2b1c3771 (old id 250031)
- alternative location
- http://www.cmj.org/Periodical/paperlist.asp?id=LW8227&linkintype=pubmed
- date added to LUP
- 2016-04-01 16:05:30
- date last changed
- 2022-01-28 17:11:50
@article{0a6fdde9-b97b-41ae-b12c-438d2b1c3771,
abstract = {{Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In order to explore the universality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii) were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA and parC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) and sequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drug efflux were analysed.}},
author = {{Shi, WF and Jiang, JP and Xu, Ning and Huang, ZM and Wang, YY}},
issn = {{0366-6999}},
keywords = {{m-chlorophenylhydrazone; carbonyl cyanide; reserpine; Acinetobacter baumanii; efflux effect; gyrA; parC}},
language = {{eng}},
number = {{4}},
pages = {{340--343}},
publisher = {{Chinese Medical Association}},
series = {{Chinese Medical Journal}},
title = {{Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii}},
url = {{http://www.cmj.org/Periodical/paperlist.asp?id=LW8227&linkintype=pubmed}},
volume = {{118}},
year = {{2005}},
}